Diosmetin suppresses human prostate cancer cell proliferation through the induction of apoptosis and cell cycle arrest

International Journal of Oncology
Christine OakSanjeev Shukla

Abstract

Diosmetin, a plant flavonoid, has been shown to exert promising effects on prostate cancer cells as an anti‑proliferative and anticancer agent. In this study, using western blot analysis for protein expression and flow cytometry for cell cycle analysis, we determined that the treatment of the LNCaP and PC‑3 prostate cancer cells with diosmetin resulted in a marked decrease in cyclin D1, Cdk2 and Cdk4 expression levels (these proteins remain active in the G0‑G1 phases of the cell cycle). These changes were accompanied by a decrease in c-Myc and Bcl-2 expression, and by an increase in Bax, p27Kip1 and FOXO3a protein expression, which suggests the potential modulatory effects of diosmetin on protein transcription. The treatment of prostate cancer cells with diosmetin set in motion an apoptotic machinery by inhibiting X-linked inhibitor of apoptosis (XIAP) and increasing cleaved PARP and cleaved caspase-3 expression levels. On the whole, the findings of this study provide an in-depth analysis of the molecular mechanisms responsible for the regulatory effects of diosmetin on key molecules that perturb the cell cycle to inhibit cell growth, and suggest that diosmetin may prove to be an effective anticancer agent for use in the treatm...Continue Reading

References

Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer
Feb 21, 2002·The Oncologist·T Sandal
Apr 3, 2002·Trends in Molecular Medicine·Edward A Sausville
Nov 24, 2004·British Journal of Cancer·C BensonJ de Bono
Apr 11, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Geoffrey I Shapiro
Feb 13, 2008·Developmental Cell·Arnaud BessonJames M Roberts
Apr 9, 2008·Journal of Cellular Biochemistry·Vidyalakshmi ChandramohanGail E Sonenshein
Nov 4, 2008·Cancer Letters·Vasilis AndroutsopoulosGerry A Potter
Sep 8, 2010·Prostate Cancer and Prostatic Diseases·D HawksworthG Petrovics
Mar 19, 2011·Cell Death and Differentiation·P N Kelly, A Strasser
Jul 28, 2011·Drug Metabolism and Pharmacokinetics·Luigi QuintieriMaura Floreani
Dec 6, 2011·Cell Death and Differentiation·E C FerberA Schulze
Jan 24, 2013·Molecular Systems Biology·Astrid EijkelenboomBoudewijn M T Burgering
May 1, 2013·Frontiers in Oncology·Barrie PeckAlmut Schulze
Sep 2, 2014·Nature Communications·Peng DongLingchong You
Sep 3, 2014·Genome Biology·Marcos Malumbres
Jan 7, 2015·Journal of Pharmaceutical and Biomedical Analysis·Enrique Barrajón-CatalánMokhtar Zarrouk
Jan 31, 2015·Nature Reviews. Drug Discovery·Uzma AsgharErik S Knudsen
Jun 11, 2015·International Journal of Oncology·Antonio BarbieriClaudio Arra
Sep 15, 2016·Future Oncology·Matteo FerroOttavio De Cobelli
Jan 4, 2017·Oncotarget·Míriam Tarrado-CastellarnauMarta Cascante
Feb 8, 2018·Molecular Pharmaceutics·Alessia RomaPaul A Spagnuolo

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Citations

Nov 23, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Amira SalehWaleed Barakat
Jun 18, 2020·Cancer Management and Research·Aiqing Ma, Rui Zhang
Jan 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuli YanYuxin Li
Jul 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sanaz KooshaMohammed A Alshawsh

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Methods Mentioned

BETA
protein
electrophoresis

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