Dipeptide synthesis by an aminopeptidase from Streptomyces septatus TH-2 and its application to synthesis of biologically active peptides

Applied and Environmental Microbiology
Jiro ArimaTadashi Hatanaka

Abstract

Dipeptide synthesis by aminopeptidase from Streptomyces septatus TH-2 (SSAP) was demonstrated using free amino acid as an acyl donor and aminoacyl methyl ester as an acyl acceptor in 98% methanol (MeOH). SSAP retained its activity after more than 100 h in 98% MeOH, and in the case of phenylalanyl-phenylalanine methyl ester synthesis, the enzyme reaction reached equilibrium when more than 50% of the free phenylalanine was converted to the product. In an investigation of the specificity of SSAP toward acyl donors and acyl acceptors, SSAP showed a broad specificity toward various free amino acids and aminoacyl methyl esters. Furthermore, we applied SSAP to the synthesis of several biologically active peptides, such as aspartyl-phenylalanine, alanyl-tyrosine, and valyl-tyrosine methyl esters.

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Citations

May 26, 2010·Applied Microbiology and Biotechnology·Jiro ArimaTadanori Aimi
Oct 13, 2010·Applied Biochemistry and Biotechnology·Arya NandanK M Nampoothiri
Jan 6, 2011·Applied Biochemistry and Biotechnology·Tadashi HatanakaYuya Kumagai
Jul 4, 2013·Applied Biochemistry and Biotechnology·Mayumi Matsushita-MoritaKen-Ichi Kusumoto
Sep 23, 2000·Molecular and Cellular Endocrinology·C M Perez-StableB A Roos
Apr 27, 2010·Applied and Environmental Microbiology·Jiro ArimaTadashi Hatanaka
Sep 29, 2011·Applied and Environmental Microbiology·Jiro ArimaNobuhiro Mori
May 4, 2006·Annual Review of Plant Biology·Hirokazu Tsukaya
Feb 28, 2008·Biochimica Et Biophysica Acta·Yoshiko UesugiTadashi Hatanaka
Sep 25, 2007·Journal of Applied Microbiology·V NagyG Szakacs
Aug 9, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Alejandro GutiérrezM Concepción Gimeno
Sep 26, 2008·Chemistry : a European Journal·Hyunjung ParkKwan Mook Kim

Related Concepts

Sodium Methoxide
Aminopeptidase
High Pressure Liquid Chromatography Procedure
Dipeptides
Streptomyces
Thermodynamics

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