Dipeptidyl peptidase-4 inhibition prevents cell death via extrinsic and intrinsic apoptotic pathways in rat pancreas with insulin resistance

Cell Biochemistry and Function
Gulay NephanSema Bolkent

Abstract

The study aims to evaluate the effect of saxagliptin, a specific inhibitor of dipeptidyl peptidase-4 enzymes, on body weight gain, lipid profiles, and cell death through apoptosis in rats with insulin resistance (IR). Male adult Sprague-Dawley rats (n = 32) were divided into 4 groups: control (Ctrl), IR, saxagliptin control, and IR treated with saxagliptin(IR + S). Insulin resistance was induced by 10% fructose in the drinking water for 8 weeks. Saxagliptin (10 mg/kg/day) was administrated by oral gavage for 2 weeks. Biochemical parameters were measured spectrophotometrically. Peptides were determined by the streptavidin-biotin-peroxidase method. Although the amount of food and liquid consumed are inversely proportional, the calories received are almost equal between both Ctrl and IR groups, as well as IR and IR + S groups. Increased homeostasis model assessment for insulin resistance, HOMA-β, triglycerides, and very low-density lipoprotein in the IR group were comparatively decreased by saxagliptin administration. The area percentage of caspase-3 and apoptotic peptidase activating factor-1 immunopositive cells in the IR + S group decreased compared with the IR group. Similarly, the percentages of caspase-8 and -9 immunopositiv...Continue Reading

References

Nov 1, 1987·Hypertension·I S HwangG M Reaven
Aug 22, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R M BezerraJ A Gontijo
Oct 30, 2001·International Journal of Radiation Biology·T PauneskuG E Woloschak
May 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stacey L ConarelloBei B Zhang
Aug 22, 2003·The Journal of Biological Chemistry·Isabelle FrouinA Ivana Scovassi
Feb 23, 2005·Nutrition & Metabolism·Heather BascianoKhosrow Adeli
Jun 13, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Singareddy Sreenivasa ReddyDesireddy Saralakumari
Nov 27, 2009·The Journal of Biological Chemistry·Naim AlkhouriAriel E Feldstein
Mar 12, 2010·Diabetes/metabolism Research and Reviews·M CiveraC Montoliu
Nov 6, 2010·Nutrition & Metabolism·Salwa W Rizkalla
Dec 21, 2010·Annals of Botany·Wojciech Strzalka, Alicja Ziemienowicz
Dec 24, 2010·Cell Communication and Signaling : CCS·Ganta Vijay ChaitanyaPhanithi Prakash Babu
Apr 19, 2011·Toxicology Mechanisms and Methods·Kimberley D Bruce, Felino R Cagampang
May 15, 2012·Pharmacological Reports : PR·Rachel D Wilson, Md Shahidul Islam
Aug 16, 2012·Clinics·Cristiano MostardaMaria Claudia Irigoyen
Sep 13, 2012·World Journal of Gastroenterology : WJG·Marta Alegret, Juan C Laguna
Dec 14, 2012·World Journal of Gastroenterology : WJG·Alba RebolloJuan C Laguna
Mar 29, 2014·PloS One·Daniël B van SchalkwijkJosé M Ordovás
Jul 22, 2014·BioMed Research International·Norshalizah MamikuttyFarihah Haji Suhaimi
Apr 4, 2015·Cold Spring Harbor Perspectives in Biology·David R McIlwainTak W Mak
May 19, 2016·Archives of Endocrinology and Metabolism·Luciana C AntunesMarcello C Bertoluci
Jul 2, 2016·Nature Reviews. Cancer·Gabriel Ichim, Stephen W G Tait
Aug 24, 2016·Nature Reviews. Rheumatology·Carla M CudaHarris Perlman
Mar 5, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Youngshim ChoiByoung-Joon Song
May 16, 2016·Journal of Clinical & Translational Endocrinology·Henry ZeladaHelard Manrique

❮ Previous
Next ❯

Citations

Apr 4, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Xinxing Guo, Jing Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis