PMID: 9425109Feb 28, 1998Paper

Dipeptidyl peptidase III is a zinc metallo-exopeptidase. Molecular cloning and expression

The Biochemical Journal
K FukasawaM Harada

Abstract

We have purified dipeptidyl peptidase III (EC 3.4.14.4) from human placenta. It had a pH optimum of 8.8 and readily hydrolysed Arg-Arg-beta-naphthylamide. Monoamino acid-, Gly-Phe-, Gly-Pro- and Bz-Arg-beta-naphthylamides were not hydrolysed at all. The enzyme was inhibited by p-chloromercuriphenylsulphonic acid, metal chelators and 3,4-dichloroisocoumarin and contained 1 mol of zinc per mol of enzyme. The zinc dissociation constant was 250 fM at pH 7. 4 as determined by the zinc binding study. We isolated, by immunological screening of a Uni-ZAP XR cDNA library constructed from rat liver mRNA species, a cDNA clone with 2633 bp encoding the rat enzyme. The longest open reading frame encodes a 827-residue protein with a theoretical molecular mass of 92790 Da. Escherichia coli SOLR cells were infected with the pBluescript phagemid containing the cloned cDNA and established the overexpression of a protein that hydrolysed Arg-Arg-beta-naphthylamide. The recombinant protein was purified and the amino acid sequence of the protein was confirmed. We presumed that the putative zinc-binding domain involved in catalysis was present in the recombinant enzyme. It was a novel zinc-binding motif in that one amino acid residue was inserted int...Continue Reading

Citations

Nov 19, 2011·Biochemical and Biophysical Research Communications·Crystal E SäbelStefan Siemann
Oct 15, 2009·Biochimie·Nina Jajcanin-JozićMarija Abramić
Jul 9, 2003·European Journal of Biochemistry·Claire MazzoccoJacques Puiroux
Jul 29, 2011·The FEBS Journal·Subhash C Prajapati, Shyam S Chauhan
Aug 4, 2011·Journal of Molecular Recognition : JMR·Antonija TomićSanja Tomić
Jul 31, 2002·Biochemical and Biophysical Research Communications·Curtis J WrayPer Olof Hasselgren
May 1, 2010·Biochimie·Neil D Rawlings
Nov 30, 2014·Journal of Endocrinological Investigation·Ž Mačak ŠafrankoM Abramić
Nov 10, 2010·Biochemical and Biophysical Research Communications·Crystal E SäbelStefan Siemann
May 27, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Ana CvitešićMarija Abramić
Feb 9, 2012·Journal of Amino Acids·Kayoko M FukasawaJunzo Hirose
Jul 29, 2016·Biological Chemistry·Zrinka KaračićMarija Abramić
Sep 11, 2018·Journal of Biomolecular Structure & Dynamics·Dejan AgićMarija Abramić
Jan 1, 2008·Biological Chemistry·Branka Salopek-SondiMarija Abramić
Sep 6, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Antonija Tomić, Sanja Tomić
Jan 31, 2013·Biological Chemistry·Nina Jajčanin-Jozić, Marija Abramić
Jan 13, 2015·Biological Chemistry·Marija AbramićNina Jajčanin-Jozić
Aug 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·E W WangR Glas
Nov 13, 2013·The Journal of Clinical Investigation·Jaba GamrekelashviliTim F Greten
Nov 5, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Junzo Hirose
Mar 7, 2007·Biological Chemistry·Marina BarsunMarija Abramić
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yanxia LiuHendrik Luesch
Dec 23, 2003·The International Journal of Biochemistry & Cell Biology·Marija AbramićLjerka Dolovcak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.