Diphenylarsinic acid increased the synthesis and release of neuroactive and vasoactive peptides in rat cerebellar astrocytes

Journal of Neuropathology and Experimental Neurology
Takayuki NegishiTomoko Tashiro

Abstract

An incident of poisoning occurred in Japan in 2003 when high-level contamination with arsenic, mainly diphenylarsinic acid (DPAA), was found in well water. People using this water particularly experienced cerebellar symptoms. In the present study, we investigated the adverse effects of DPAA on the cerebellum in vitro and in vivo to understand the biological mechanisms that cause cerebellar symptoms. Comprehensive gene expression analyses in primary cultured ratcerebellar cells exposed to 10 μM DPAA for 24 hours indicated significant alterations in the mRNA expression of genes encoding antioxidative stress proteins (heme oxigenase 1 and heat shock protein72) and neuroactive and vasoactive peptides (neuropeptide Y, adrenomedullin, monocyte chemoattractant protein 1, and fibroblast growth factor 2). Further analyses of proteins revealed that cultured cerebellar astrocytes expressed these antioxidative stress proteins and peptides in response to exposure to DPAA. In addition, these adverseeffects were also observed in the cerebellum exposed in vivo to DPAA (100 mg/L) for 21 days. These results suggested that cerebellarastrocytes irregularly secrete neuroactive and vasoactive peptidesagainst DPAA-induced oxidative stress, which lead...Continue Reading

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Citations

Sep 24, 2013·Biological Trace Element Research·Xiaona LiuJing Wang
Feb 11, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kazuhiro IshiiAkira Tamaoka
Jul 6, 2014·Neurochemistry International·Nimesha TadepalleRalf Dringen
Jan 29, 2016·International Journal of Molecular Sciences·Huai GuanFengyuan Piao
Aug 23, 2018·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·K IshiiA Tamaoka
Nov 22, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Carla Garza-LombóRodrigo Franco
Feb 6, 2021·Environmental Toxicology and Pharmacology·Xiaona LiuJing Wang
May 6, 2021·Molecular Biology Reports·Elif OguzSaniye Ada

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