Dipolar rearrangement during micellization explored using a potential-sensitive fluorescent probe

Chemistry and Physics of Lipids
Parijat Sarkar, Amitabha Chattopadhyay

Abstract

Dipole potential is the potential difference within the membrane bilayer, which originates due to the nonrandom arrangement of lipid dipoles and water molecules at the membrane interface. Although dipole potential is generally used in the context of bilayer membranes, the nonrandom arrangement of amphiphiles and water dipoles would also contribute to dipole potential in organized molecular assemblies such as micelles. In this work, we show that the process of micelle formation from monomers for a representative variety of detergents is associated with dipolar rearrangement. We monitor the dipolar reorganization upon micellization as a change in dipole potential, measured by the dual wavelength ratiometric approach utilizing the potential-sensitive membrane probe di-8-ANEPPS. We further utilized this phenomenon to estimate the critical micelle concentration (CMC) of a variety of detergents. CMC determined by this method are in overall agreement with the literature values of CMC for these detergents. To the best of our knowledge, these results constitute the first report showing dipolar reorientation during micellization. We conclude that dipole potential measurements could provide a novel approach to explore micellar organization.

References

Mar 25, 1975·Biochimica Et Biophysica Acta·A Helenius, K Simons
Oct 1, 1972·Archives of Internal Medicine·M C Carey, D M Small
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L M Hjelmeland
Sep 6, 1994·Chemistry and Physics of Lipids·H BROCKMAN
Jan 31, 1997·Biochimica Et Biophysica Acta·R J Clarke, D J Kane
Feb 24, 2001·Advances in Colloid and Interface Science·R J Clarke
Apr 9, 2004·European Biophysics Journal : EBJ·H Raghuraman, Amitabha Chattopadhyay
Mar 4, 2006·Biophysical Journal·Thomas Starke-PeterkovicRonald J Clarke
Apr 20, 2006·Chemistry and Physics of Lipids·Noriaki FunasakiShun Hirota
Dec 21, 2006·Biochimica Et Biophysica Acta·Gaëlle Le GoffRonald J Clarke
Jan 10, 2009·European Biophysics Journal : EBJ·Thomas Starke-Peterkovic, Ronald J Clarke
Oct 17, 2009·Biochemical and Biophysical Research Communications·Arunima ChaudhuriAmitabha Chattopadhyay
Mar 24, 2011·The Journal of Physical Chemistry. B·David RobinsonJonathan D Hirst
Mar 27, 2012·Annual Review of Biochemistry·Liguo Wang
Apr 17, 2012·Biophysical Journal·Sourav HaldarAmitabha Chattopadhyay
Dec 4, 2012·Biochimica Et Biophysica Acta·Pushpendra SinghAmitabha Chattopadhyay
Mar 22, 2014·The Journal of Physical Chemistry. B·Fernando E HerreraDaniel E Rodrigues
May 8, 2014·Biochimica Et Biophysica Acta·Arunima Chaudhuri, Amitabha Chattopadhyay
Sep 16, 2014·Chemistry and Physics of Lipids·Suman BandariAmitabha Chattopadhyay
Mar 31, 2015·Biochimica Et Biophysica Acta·Joanna L RichensPaul O'Shea
Aug 6, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Ramon Barnadas-Rodríguez, Josep Cladera

❮ Previous
Next ❯

Citations

Dec 1, 2015·Chemistry and Physics of Lipids·Parijat Sarkar, Amitabha Chattopadhyay
Feb 17, 2019·The Journal of Biological Chemistry·Alvaro GarciaRonald J Clarke
Jul 2, 2017·Scientific Reports·Parijat SarkarAmitabha Chattopadhyay

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.