Direct acridine orange fluorescence examination of blood slides compared to current techniques for malaria diagnosis

Transactions of the Royal Society of Tropical Medicine and Hygiene
F GayA Fribourg-Blanc

Abstract

The renewed interest in the use of fluorochromes for malaria diagnosis prompted us to evaluate the acridine orange fluorescence technique on blood slides, and to compare it with established techniques using thick and thin blood films and the QBC malaria test, using the Giemsa-stained thick film technique as our standard method for comparison. We compared 123 positively diagnosed cases and 120 negative cases. For primary samples (day 0), the sensitivity of the thin blood film fluorescence acridine orange technique (AO) was 96.4%, and its specificity was 95.1%. In cases of imported malaria, with a prevalence rate of 16.2%, the positive predictive value was 79.2% and the negative predictive value 99.3%. Sensitivity of AO was significantly higher than that of Giemsa-stained thin blood films for parasitaemias < 5000/microL. The potential of AO for species diagnosis of Plasmodium was 85.2%, using Giemsa-stained thin films as the reference technique. Where QBC imposes a cost limitation, especially in developing countries, despite its high performance, the AO diagnostic technique is a valuable alternative, because of its simplicity, almost negligible cost, and its diagnostic reliability. The method may also have potential value in the ...Continue Reading

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Citations

Jan 1, 2004·Indian Journal of Clinical Biochemistry : IJCB·M M MyaD N Rao
Dec 3, 1999·Clinical and Laboratory Haematology·T Hänscheid
Sep 30, 2000·Clinical and Laboratory Haematology·A H Moody, P L Chiodini
May 12, 2010·Veterinary Clinical Pathology·Mehrdad Ameri
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Feb 9, 2018·Malaria Journal·Masatsugu KimuraAkira Kaneko
Apr 5, 2011·The Korean Journal of Parasitology·Wanna ChaijaroenkulKesara Na-Bangchang
Mar 13, 2013·Expert Review of Molecular Diagnostics·Eun-Taek Han
Aug 11, 2020·Expert Review of Anti-infective Therapy·Rosauro VaroQuique Bassat
Sep 10, 2020·Microorganisms·Muneaki HashimotoMasatoshi Kataoka
Dec 17, 1998·Journal of Clinical Microbiology·L CiceronM Danis

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