Direct activation of human peritoneal mesothelial cells by heat-killed microorganisms

Annals of Surgery
P KinnaertI Salmon

Abstract

The aim of the study was to determine if human peritoneal mesothelial cells (HPMCs) can be activated directly by bacterial products contained in preparations of heat-killed Escherichia coli and staphylococci. It has been shown recently that cytokine-activated HPMCs produce the inflammatory mediators, interleukin-1, interleukin-6, interleukin-8, and macrophage chemotactic protein-1. Studies concerning the effects of bacterial products on HPMCs are scarce and have not yielded conclusive results. Growth-arrested HPMC monolayers were prepared from cell suspensions obtained by enzymatic disaggregation of small pieces of omentum. They were incubated for 24 hours with heat-killed E. coli (ATCC 25922), heat-killed staphylococci (ATCC 25933), or E. coli lipopolysaccharide, and the release of various cytokines in the culture media was measured by radioimmunoassays or enzyme-linked immunosorbent assays. Results were expressed as mean +/- standard error of the mean in picograms per milliliter of supernatant and analyzed with the Wilcoxon test; p values of less than 0.05 were considered significant. Baseline production of interleukin-6, interleukin-8, the chemokine "regulated upon activation, normal T cell expressed and secreted" (RANTES), ...Continue Reading

References

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Citations

Aug 1, 1997·Current Opinion in Immunology·N W Schluger, W N Rom
Nov 20, 2004·ANZ Journal of Surgery·Veronica YaoJohn C Hall
Apr 16, 1998·The Journal of Surgical Research·P K HenkeJ C Peyton
Oct 28, 2010·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Elyamani FoudaMohamed Farid
May 16, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Petra GaisBernhard Holzmann
Mar 29, 2001·Journal of the American Society of Nephrology : JASN·Anna BasokAmos Douvdevani

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