Direct and fast determination of antiretroviral drugs by automated online solid-phase extraction-liquid chromatography-tandem mass spectrometry in human plasma

Clinical Chemistry and Laboratory Medicine : CCLM
Therese KoalVolkhard Kaever

Abstract

In this study antiretroviral drugs of the classes protease inhibitors (PI) and non-nucleoside reverse transcriptase inhibitors (NNRTI) were quantified for the first time directly in patient plasma samples by means of an automated and validated online solid-phase extraction-liquid chromatography-tandem mass spectrometry (XLC-MS/MS) method using the Symbiosis Pharma system (Spark Holland) for XLC coupled to an API 2000 for MS/MS analysis. The PI drugs amprenavir, nelfinavir, indinavir, lopinavir, saquinavir, ritonavir, and atazanavir, and the NNRTI drugs nevirapine and efavirenz in real patient samples were analysed in a 25-microL sample volume, which was only diluted with 200 microL of H2O (containing 500 ng/mL of the internal standard reserpine) to minimise the matrix concentration and to add the internal standard. No additional tedious and time-consuming sample preparation steps such as protein precipitation, centrifugation, and pipetting were per-formed for sample clean-up. The high-throughput method developed allowed the simultaneous analysis of two samples (first analysis 6.6 min, subsequent analyses 3.3 min between injections) and has been validated in terms of the limit of detection (LOD, 2-70 ng/mL), lower limit of quant...Continue Reading

References

May 15, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Michael DetersVolkhard Kaever
Apr 23, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Katharina M Rentsch
Jun 21, 2003·Clinical Chemistry·Thomas M Annesley
Mar 10, 2004·AIDS Patient Care and STDs·Natella Y RakhmaninaSteven J Soldin
May 12, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Therese KoalVolkhard Kaever
Oct 29, 2004·Clinical Pharmacokinetics·Bregt S KappelhoffJos H Beijnen
May 10, 2005·Antiviral Research·Jindrich CinatlHans Wilhelm Doerr
Sep 30, 2005·Rapid Communications in Mass Spectrometry : RCM·Therese KoalVolkhard Kaever

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Citations

Mar 2, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Elena Llorente FernándezJordi To-Figueras
Jan 12, 2008·Journal of Pharmaceutical Sciences·Maria M TotevaDrazen Ostovic
Jul 6, 2010·Clinical Biochemistry·Michael Vogeser, Fabian Kirchhoff
Apr 1, 2008·Clinical Biochemistry·Michael Vogeser, Christoph Seger
Dec 15, 2017·Biomedical Chromatography : BMC·Timothy L ArchibaldStacy D Brown

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