Direct and indirect roles of cytochrome b in the mediation of superoxide generation and NO catabolism by mitochondrial succinate-cytochrome c reductase.

The Journal of Biological Chemistry
Yeong-Renn ChenJay L Zweier

Abstract

Mitochondrial superoxide (O2*-) production is an important mediator of oxidative cellular injury. Succinate-cytochrome c reductase (SCR) of the electron transport chain has been implicated as an essential part of the mediation of O2*- generation and an alternative target of nitric oxide (NO) in the regulation of mitochondrial respiration. The Q cycle mechanism plays a central role in controlling both events. In the present work, O2*- generation by SCR was measured with the EPR spin-trapping technique using DEPMPO (5-diethoxylphosphoryl-5-methyl-1-pyrroline N-oxide) as the spin trap. In the presence of succinate, O2*- generation from SCR was detected as the spin adduct DEPMPO/*OOH. Inhibitors of the Q(o*-) site only marginally reduced (20-30%) this O2*- production, suggesting a secondary role of Q(o*-) in the mediation of O2*- generation. Addition of cyanide significantly decreased (approximately 70%) O2*- production, indicating the involvement of the heme component. UV-visible spectral analysis revealed that oxidation of ferrocytochrome b was accompanied by cytochrome c(1) reduction, and the reaction was mediated by the formation of an O2*- intermediate, indicating a direct role for cytochrome b in O2*- generation. In the prese...Continue Reading

References

Mar 1, 1985·Archives of Biochemistry and Biophysics·J F TurrensA L Lehninger
Jul 31, 1986·Biochemical and Biophysical Research Communications·H Nohl, W Jordan
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J L ZweierM L Weisfeldt
Nov 1, 1980·The Biochemical Journal·J F Turrens, A Boveris
Jun 1, 1994·Journal of Magnetic Resonance. Series B·D R Duling
Jan 1, 1993·Free Radical Research Communications·R E Huie, S Padmaja
Jul 1, 1996·Archives of Biochemistry and Biophysics·R WelterC A Yu
May 16, 1998·Nature·Z ZhangS H Kim
Jul 21, 1998·The Journal of Biological Chemistry·X LiuJ R Lancaster
May 8, 1999·Free Radical Biology & Medicine·J J PoderosoE Cadenas
Dec 23, 1999·The Journal of Biological Chemistry·J J PoderosoA Boveris
Mar 29, 2000·The Journal of Biological Chemistry·C I LeeJ L Zweier
Oct 26, 2000·Trends in Biochemical Sciences·S Raha, B H Robinson
Mar 10, 2001·Free Radical Research·E CadenasA Boveris
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·S ShivaV M Darley-Usmar
May 8, 2002·Nature Reviews. Molecular Cell Biology·Salvador Moncada, Jorge D Erusalimsky
Nov 26, 2002·Free Radical Biology & Medicine·Anup RamachandranVictor Darley-Usmar
Feb 8, 2003·Science·Enzo NisoliMichele O Carruba
Oct 17, 2003·The Journal of Physiology·Julio F Turrens
Nov 1, 2003·Archives of Biochemistry and Biophysics·Jian Sun, Bernard L Trumpower
Nov 20, 2003·The Journal of Biological Chemistry·Alexei P KudinWolfram S Kunz
Feb 6, 2004·The Journal of Biological Chemistry·Yeong-Renn ChenRonald P Mason
May 8, 2004·Antioxidants & Redox Signaling·Frederick A Villamena, Jay L Zweier
Dec 24, 2004·The Journal of Biological Chemistry·Xing GongChang-An Yu
Jun 21, 2005·Archives of Biochemistry and Biophysics·Yeong-Renn ChenJay L Zweier

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Citations

Jun 15, 2007·Journal of the American Chemical Society·Frederick A VillamenaJay L Zweier
Nov 13, 2010·Journal of the American Chemical Society·Shang-U KimFrederick A Villamena
Sep 12, 2007·The Journal of Physical Chemistry. a·Frederick A VillamenaJay L Zweier
Jul 16, 2008·The Journal of Biological Chemistry·Chun-An ChenJay L Zweier
Jan 10, 2009·The Journal of Pharmacology and Experimental Therapeutics·Zhaosheng HanB Rita Alevriadou
May 16, 2008·American Journal of Physiology. Cell Physiology·Charles I JonesB Rita Alevriadou
Nov 15, 2011·Circulation Journal : Official Journal of the Japanese Circulation Society·Kyung-Sun HeoJun-ichi Abe
Jun 20, 2012·Future Medicinal Chemistry·Frederick A VillamenaKevin M Nash
Jun 16, 2009·FEBS Letters·Halley Caixeta OliveiraIone Salgado
Aug 10, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Kevin M Nash, Salahuddin Ahmed
Oct 5, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sachin KumarMadhu Dikshit
Mar 17, 2011·Biochimica Et Biophysica Acta·Jingfeng ChenYeong-Renn Chen
Mar 29, 2008·Biochimica Et Biophysica Acta·Halley Caixeta de OliveiraIone Salgado
Jan 1, 2012·Scientifica·Paul R Gardner
May 21, 2016·Oxidative Medicine and Cellular Longevity·Danina M MunteanCristian Mornoș
Jan 25, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sibel SuzenLuciano Saso
Jul 16, 2016·Circulation Research·Kathy K GriendlingUNKNOWN American Heart Association Council on Basic Cardiovascular Sciences
Feb 1, 2014·Circulation Research·Yeong-Renn Chen, Jay L Zweier
Aug 31, 2011·Critical Reviews in Clinical Laboratory Sciences·Ivan Spasojević
Sep 13, 2007·The Journal of Biological Chemistry·Yeong-Renn ChenJay L Zweier
Mar 12, 2008·The Journal of Organic Chemistry·Yongbin HanFrederick A Villamena

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