Direct and mediated electrochemical response of the cytochrome P450 106A2 from Bacillus megaterium ATCC 13368

Bioelectrochemistry
Hélène ColasPedro de Oliveira

Abstract

CYP106A2 is one of only a few known steroid hydroxylases of bacterial origin, which might be interesting for biotechnological applications. Despite the enzyme having been studied for more than 30 years, its physiological function remains elusive. To date, there have been no reports of the redox potential of CYP106A2, which was supposed to be unusually low for a cytochrome P450. In this work we show that cyclic voltammetry is not only suitable to determine the redox potential of challenging proteins such as CYP106A2, measured at -128 mV vs. NHE, but also to study molecular interactions of the enzyme with different interaction partners via the respective electrochemical responses. The effect of small ligands, such as carbon monoxide and cyanide, was observed on the cyclic voltammograms of CYP106A2. Furthermore, we found that Tween 80 caused a positive shift of the redox potential of immobilised CYP106A2 indicative for water expulsion from the haem environment. Moreover, electron transfer mediation phenomena with biological redox partners (e.g. ferredoxins) were studied. Finally, the influence of two different kinds of substrates on the electrochemical response of CYP106A2 was assessed, aligning observations from spectral and elec...Continue Reading

References

Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S G Sligar, I C Gunsalus
Nov 16, 1992·Biochemical and Biophysical Research Communications·H UhlmannR Bernhardt
Feb 19, 2000·Biochemical and Biophysical Research Communications·C LeiF W Scheller
Feb 24, 2001·Faraday Discussions·S J SadeghiG Gilardi
May 2, 2002·Biochimica Et Biophysica Acta·Christiane Jung
Nov 25, 2003·Biochemical and Biophysical Research Communications·Christiane JungGaston Hui Bon Hoa
Jan 27, 2004·Biochemical and Biophysical Research Communications·Nikitas BistolasUlla Wollenberger
Jun 9, 2005·Chemical Reviews·Ilia G DenisovIlme Schlichting
Dec 6, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Smilja TodorovicDaniel H Murgida
Mar 7, 2006·Journal of Biotechnology·Rita Bernhardt
Mar 16, 2006·Chemical Communications : Chem Comm·Andrea FantuzziGianfranco Gilardi
Aug 3, 2006·Journal of the American Chemical Society·Andrew K UditMichael G Hill
Sep 19, 2006·Biochimica Et Biophysica Acta·Frank HannemannRita Bernhardt
Nov 1, 2006·Biochemical Society Transactions·C VirusR Bernhardt
Jul 29, 2009·Biochimica Et Biophysica Acta·Guillermina GoñiMilagros Medina
Jun 12, 2010·Biochimica Et Biophysica Acta·Kerstin Maria EwenRita Bernhardt
Jul 22, 2010·Biochimica Et Biophysica Acta·Sheila J SadeghiGianfranco Gilardi
Jan 29, 2011·Chembiochem : a European Journal of Chemical Biology·Sabrina BleifRita Bernhardt

❮ Previous
Next ❯

Citations

Sep 10, 2015·Biomedit︠s︡inskai︠a︡ khimii︠a︡·O V GnedenkoA I Archakov
Apr 23, 2014·Biomedit︠s︡inskai︠a︡ khimii︠a︡·O V GnedenkoA I Archakov
Sep 16, 2018·Biosensors & Bioelectronics·Victoria V ShumyantsevaAlexander I Archakov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.