Direct and simultaneous identification of Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) by rapid multiplex nested PCR-ICT assay

Journal of Microbiological Methods
Po-Chi SooHsin-Chih Lai

Abstract

The Mycobacterium tuberculosis (MTB) shows different virulence and host infection range from other members of the M. tuberculosis complex (MTBC). Differential identification of MTB from MTBC is thus important in certain occasions. The currently commercially available molecular assays which use either IS6110 or 16S rDNA fragment as identification targets are mainly designed for identifying MTBC but not for MTB. Comparative genomic DNA analysis has provided valuable information on regions of difference (RD) present in MTB but not in other members of the MTBC. RD9 region is further suggested to be a potential target for differential identification of MTB from MTBC. In this study, using IS6110 and Rv3618 (belong to RD9) as the specific identification targets for MTBC and MTB, respectively, we developed and tested a multiplex nested PCR-ICT (immuno-chromatography test) assay for simultaneously and directly detecting not only MTBC but also MTB from 1500 clinical sputum specimens. The results were compared with traditional culture and biochemical identification results together with patients' clinical assessments. This assay showed a 95.5% sensitivity, 97.9% specificity, 2.1% false positive rate and 4.5% false negative rate towards de...Continue Reading

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Citations

Aug 13, 2008·Analytical and Bioanalytical Chemistry·Geertruida A Posthuma-TrumpieAart van Amerongen
Jan 11, 2013·The American Journal of Tropical Medicine and Hygiene·Elizabeth De Francesco DaherElvino José Guardão Barros
Nov 22, 2013·BioMed Research International·Sara MacenteRosilene Fressatti Cardoso
May 15, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Chi-Shih ChuYao-Chi Su
May 22, 2008·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Chia-Chen Lu, Hsin-Chih Lai
Aug 19, 2016·The Journal of Veterinary Medical Science·Zhang QuanZhu Hongfei
Nov 1, 2016·The Journal of Microbiology·Hsin-Chih LaiPo-Chi Soo
Aug 29, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mohammad Khaja Mafij UddinSayera Banu

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