Abstract
The higher plant ADP-glucose pyrophosphorylase is a heterotetramer consisting of two subunit types, which have evolved at different rates from a common ancestral gene. The potato tuber small subunit (SS) displays both catalytic and regulatory properties, whereas the exact role of the large subunit (LS), which contains substrate and effector binding sites, remains unresolved. We identified a mutation, S302N, which increased the solubility of the recombinant potato tuber LS and, in turn, enabling it to form a homotetrameric structure. The LS302N homotetramer possesses very little enzyme activity at a level 100-fold less than that seen for the unactivated SS homotetramer. Unlike the SS enzyme, however, the LS302N homotetramer enzyme is neither activated by the effector 3-phosphoglycerate nor inhibited by P(i). When combined with the catalytically silenced SS, S D143N, however, the LS302N-containing enzyme shows significantly enhanced catalytic activity and restored 3-PGA activation. This unmasking of catalytic and regulatory potential of the LS is conspicuously evident when the activities of the resurrected L(K41R.T51K.S302N) homotetramer are compared with its heterotetrameric form assembled with S D143N. Overall, these results in...Continue Reading
References
Mar 10, 1995·Molecular & General Genetics : MGG·U La CognataB Müller-Röber
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·T W GreeneT W Okita
Jul 15, 1998·Plant Physiology·Y FuJ Preiss
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·T W GreeneT W Okita
Sep 11, 1998·Plant Physiology·M A BallicoraJ Preiss
Sep 17, 1998·The Journal of Biological Chemistry·Y FuJ Preiss
Aug 3, 1999·The EMBO Journal·K BrownY Bourne
Nov 11, 1999·Plant Physiology·D N DoanO A Olsen
Jan 8, 2000·The Journal of Biological Chemistry·M A BallicoraJ Preiss
Jun 29, 2000·Trends in Plant Science·C J SlatteryT W Okita
Oct 6, 2000·FEBS Letters·P R SalamoneT W Okita
Dec 16, 2000·The EMBO Journal·W BlankenfeldtJ H Naismith
May 23, 2001·Archives of Biochemistry and Biophysics·D F Gómez-CasatiA A Iglesias
Sep 22, 2001·The Journal of Biological Chemistry·J B FrueaufJ Preiss
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Peter R SalamoneThomas W Okita
Sep 7, 2002·The Plant Cell·Axel TiessenPeter Geigenberger
Feb 13, 2003·The Plant Journal : for Cell and Molecular Biology·Jeremiah B FrueaufJack Preiss
Sep 16, 2003·Plant Physiology·Janneke H M HendriksPeter Geigenberger
Oct 2, 2003·Planta·Brian T BurgerL Curtis Hannah
May 4, 2004·Plant Physiology·Joanna M CrossL Curtis Hannah
Oct 13, 2004·Protein Expression and Purification·Seon-Kap HwangThomas W Okita
Jan 6, 2005·The Journal of Biological Chemistry·Miguel A BallicoraJack Preiss
Feb 5, 2005·The EMBO Journal·Xiangshu JinJames H Geiger
Feb 16, 2005·FEBS Letters·Seon-Kap HwangThomas W Okita
May 3, 2005·Journal of Experimental Botany·Peter GeigenbergerAxel Tiessen
Jul 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Anna KolbePeter Geigenberger
Oct 18, 2005·Photosynthesis Research·Miguel A BallicoraJack Preiss
Jun 1, 1990·Plant Physiology·T W OkitaJ Preiss
Dec 2, 2006·FEBS Letters·Seon-Kap HwangThomas W Okita
Jan 9, 2007·Phytochemistry·Seon-Kap HwangThomas W Okita
May 15, 2007·The Plant Cell·Nikolaos GeorgelisL Curtis Hannah
Citations
Sep 10, 2009·The Journal of Biological Chemistry·Misty L KuhnMiguel A Ballicora
Jul 11, 2008·Plant Physiology·Tiziana VentrigliaJosé M Romero
Jul 25, 2009·Plant Physiology·Nikolaos GeorgelisL Curtis Hannah
Oct 31, 2009·PLoS Computational Biology·Ibrahim BarisIbrahim Halil Kavakli
Apr 1, 2014·Plant Molecular Biology·Kelly A DoroshenkThomas W Okita
Apr 22, 2014·Plant & Cell Physiology·Aytug TuncelHikaru Satoh
Sep 11, 2013·Bioscience, Biotechnology, and Biochemistry·Shinji WakutaHirokazu Matsui
Jun 4, 2014·Plant & Cell Physiology·A Bengisu SeferogluI Halil Kavakli
May 9, 2015·FEBS Letters·Bilal CakirChulHee Kang
Jul 19, 2011·Plant Science : an International Journal of Experimental Plant Biology·Kelly GibsonThomas W Okita
Sep 11, 2014·The FEBS Journal·Aytug TuncelThomas W Okita
Oct 27, 2015·Molecular Genetics and Genomics : MGG·Yu-Xi ZhouHai-Yan Wang
Mar 20, 2010·The Biochemical Journal·Marina PetreikovArthur A Schaffer
Mar 19, 2013·Plant Science : an International Journal of Experimental Plant Biology·Ayse Bengisu SeferogluIbrahim Halil Kavakli
Oct 3, 2014·BioMed Research International·Kishore SarmaMahendra K Modi
Jun 15, 2016·Plant Science : an International Journal of Experimental Plant Biology·Xiao-Jie TangJian-Min Wan
Oct 9, 2016·Plant Science : an International Journal of Experimental Plant Biology·Ayse Bengisu SeferogluIbrahim Halil Kavakli
Apr 4, 2017·Plant Biotechnology Journal·Jian HouXueyong Zhang
May 19, 2018·Protoplasma·Asena GorenIan J Tetlow
Feb 26, 2019·Frontiers in Plant Science·Seon-Kap HwangThomas W Okita
Aug 10, 2016·The Journal of Biological Chemistry·Seon-Kap HwangThomas W Okita
Jan 5, 2010·Archives of Biochemistry and Biophysics·Seon-Kap HwangThomas W Okita