Direct association with and dephosphorylation of Jak2 kinase by the SH2-domain-containing protein tyrosine phosphatase SHP-1.

Molecular and Cellular Biology
H JiaoT Yi

Abstract

SHP-1 is an SH2-containing cytoplasmic tyrosine phosphatase that is widely distributed in cells of the hematopoietic system. SHP-1 plays an important role in the signal transduction of many cytokine receptors, including the receptor for erythropoietin, by associating via its SH2 domains to the receptors and dephosphorylating key substrates. Recent studies have suggested that SHP-1 regulates the function of Jak family tyrosine kinases, as shown by its constitutive association with the Tyk2 kinase and the hyperphosphorylation of Jak kinases in the motheaten cells that lack functional SHP-1. We have examined the interactions of SHP-1 with two tyrosine kinases activated during engagement of the erythropoietin receptor, the Janus family kinase Jak-2 and the c-fps/fes kinase. Immunoblotting studies with extracts from mouse hematopoietic cells demonstrated that Jak2, but not c-fes, was present in anti-SHP-1 immunoprecipitates, suggesting that SHP-1 selectively associates with Jak2 in vivo. Consistent with this, when SHP-1 was coexpressed with these kinases in Cos-7 cells, it associated with and dephosphorylated Jak2 but not c-fes. Transient cotransfection of truncated forms of SHP-1 with Jak2 demonstrated that the SHP-1-Jak2 interacti...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J PlutzkyR D Rosenberg
Aug 4, 1995·The Journal of Biological Chemistry·A YetterL C Platanias
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·O SilvennoinenJ N Ihle
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D PeiC T Walsh
Nov 1, 1995·Trends in Biochemical Sciences·Z Songyang, L C Cantley

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Citations

Sep 29, 2020·F1000Research·Senthil Velan BhoopalanMitchell J Weiss
Feb 25, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·R J Duhé, W L Farrar
Oct 6, 1998·Annual Review of Biochemistry·G R StarkR D Schreiber
Mar 24, 1999·Current Opinion in Hematology·J L Fernández-Luna
Jun 3, 1999·Clinical Chemistry and Laboratory Medicine : CCLM·M Blery, E Vivier
Mar 11, 2000·American Journal of Physiology. Cell Physiology·W P DirksenS A Fisher
Oct 3, 1999·The Journal of Biological Chemistry·M ThangarajuC B Srikant
Oct 27, 2001·The Journal of Biological Chemistry·S SarkarS Pestka
Apr 20, 2001·The Journal of Biological Chemistry·V LukashovaJ Stanková
Jan 31, 2003·Molecular and Cellular Biology·Stéphane PelletierSylvain Meloche
May 20, 2003·Immunological Reviews·Hans-Uwe Simon
Nov 19, 2003·Journal of Cellular Biochemistry·Chengyu WuG Wayne Zhou
Jan 15, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David W Sternberg, D Gary Gilliland
Mar 23, 2004·Annual Review of Immunology·Warren S Alexander, Douglas J Hilton
Jul 23, 2004·The Journal of Biological Chemistry·Matthew L JonesAlastair W Poole
Mar 1, 2006·Expert Opinion on Biological Therapy·Rebecca J LeemanJennifer Rubin Grandis
May 24, 2006·The Journal of Biological Chemistry·Jie XuJoseph L Messina
Oct 4, 2006·Nature Reviews. Drug Discovery·Christopher A McCullochHani El-Gabalawy
Oct 16, 2007·Oncogene·S J BakerE P Reddy
Aug 30, 2007·The Journal of Biological Chemistry·Leah RiderMaria Diakonova
Jan 23, 2008·Laboratory Investigation; a Journal of Technical Methods and Pathology·George P ChristophiPaul T Massa
Sep 4, 2009·Journal of Cellular Biochemistry·Nadia Mehebik-MojaatAnne-Marie Jaubert
Nov 26, 2011·American Journal of Physiology. Cell Physiology·Jayshree MishraNarendra Kumar
May 4, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Caroline LeblancNathalie Rivard
Sep 16, 2017·Acta Biochimica Et Biophysica Sinica·Jian WangGuoqiang Xing
Mar 15, 2018·International Journal of Molecular Sciences·Taku KuwabaraMotonari Kondo
Jul 1, 2008·Expert Review of Endocrinology & Metabolism·Louise T DalgaardJens H Nielsen
Jan 27, 1998·The Journal of Biological Chemistry·S O KimS J Frank

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