Direct biochemical evidence for the utilization of UDP-bacillosamine by PglC, an essential glycosyl-1-phosphate transferase in the Campylobacter jejuni N-linked glycosylation pathway

Biochemistry
Kerney Jebrell GloverBarbara Imperiali

Abstract

Campylobacter jejuni has a general N-linked glycosylation pathway, encoded by the pgl gene cluster. In C. jejuni, a heptasaccharide is transferred from an undecaprenyl pyrophosphate donor [GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac-alpha1-PP-undecaprenyl, where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose)] to the asparagine side chain of target proteins at the Asn-X-Ser/Thr motif. In this study, we have cloned, overexpressed in Escherichia coli, and purified PglC, the glycosyl-1-phosphate transferase responsible for the first step in the biosynthesis of the undecaprenyl-linked heptasaccharide donor. In addition, we report the first synthetic route to uridine 5'-diphosphobacillosamine. Using the uridine 5'-diphosphobacillosamine and undecaprenyl phosphate, we demonstrate the ability of PglC to produce undecaprenyl pyrophosphate bacillosamine using radiolabeled HPLC and mass spectral analysis. In addition, we revealed that PglC does not accept uridine 5'-diphospho-N-acetylglucosamine or uridine 5'-diphospho-N-acetylgalactosamine as substrates but will accept uridine 5'-diphospho-6-hydroxybacillosamine, an analogue of bacillosamine that retains the C-6 hydroxyl ...Continue Reading

Citations

May 8, 2010·Biotechnology Letters·Jagroop Pandhal, Phillip C Wright
Apr 22, 2011·Biochemistry·Angelyn Larkin, Barbara Imperiali
Oct 16, 2010·Nature Reviews. Microbiology·Harald Nothaft, Christine M Szymanski
Jul 5, 2012·The Journal of Biological Chemistry·Nichollas E ScottStuart J Cordwell
Jan 19, 2013·The Journal of Biological Chemistry·Harald Nothaft, Christine M Szymanski
Mar 6, 2012·Journal of Bacteriology·Adrian J JervisDennis Linton
Jun 29, 2010·Journal of Bacteriology·Adrian J JervisDennis Linton
Jul 17, 2007·Journal of Bacteriology·Patricia GuerrySusan M Logan
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jeremy J GilbreathDavid R Hendrixson
Mar 22, 2014·Journal of Bioscience and Bioengineering·Akkaraphol SrichaisupakitKazuhito Fujiyama
Dec 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Meredith D HartleyBarbara Imperiali
Nov 19, 2011·Archives of Biochemistry and Biophysics·Meredith D Hartley, Barbara Imperiali
Aug 19, 2008·Trends in Microbiology·Patricia Guerry, Christine M Szymanski
Dec 15, 2015·Chemistry : a European Journal·Marthe T C WalvoortBarbara Imperiali
Apr 1, 2008·Bioorganic & Medicinal Chemistry·Meredith D HartleyBarbara Imperiali
Oct 16, 2007·Current Opinion in Chemical Biology·Yasunori Chiba, Yoshifumi Jigami
Jun 12, 2013·Biopolymers·Ritika DwivediChristine M Szymanski
Jul 20, 2012·Protein Science : a Publication of the Protein Society·Sarah E Furlong, Miguel A Valvano
Jun 21, 2013·Molecular Microbiology·Robert G Lees-MillerMario F Feldman
Jul 3, 2013·Bioorganic & Medicinal Chemistry·Anahita Z MostafaviJerry M Troutman
Aug 24, 2010·Chemical Communications : Chem Comm·Danielle H DubeBo Wang
Oct 1, 2016·Future Microbiology·Hong LiMohammed Benghezal
Jun 21, 2017·Proceedings of the National Academy of Sciences of the United States of America·Debasis DasBarbara Imperiali
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Hanne L P Tytgat, Sarah Lebeer
Feb 2, 2018·Nature Communications·Ana S RamírezKaspar P Locher

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.