Direct biosynthesis of adipic acid from lignin-derived aromatics using engineered Pseudomonas putida KT2440.

Metabolic Engineering
Wei NiuJiantao Guo

Abstract

Lignin is one largely untapped natural resource that can be exploited as a raw material for the bioproduction of value-added chemicals. Meanwhile, the current petroleum-based process for the production of adipic acid faces sustainability challenges. Here we report the successful engineering of Pseudomonas putida KT2440 strain for the direct biosynthesis of adipic acid from lignin-derived aromatics. The devised bio-adipic acid route features an artificial biosynthetic pathway that is connected to the endogenous aromatics degradation pathway of the host at the branching point, 3-ketoadipoyl-CoA, by taking advantage of the unique carbon skeleton of this key intermediate. Studies of the metabolism of 3-ketoadipoyl-CoA led to the discovery of crosstalk between two aromatics degradation pathways in KT2440. This knowledge facilitated the formulation and implementation of metabolic engineering strategies to optimize the carbon flux into the biosynthesis of adipic acid. By optimizing pathway expression and cultivation conditions, an engineered strain AA-1 produced adipic acid at 0.76 g/L and 18.4% molar yield under shake-flask conditions and 2.5 g/L and 17.4% molar yield under fermenter-controlled conditions from common aromatics that c...Continue Reading

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Citations

Aug 14, 2020·Applied Microbiology and Biotechnology·Anna WeimerChristoph Wittmann
Aug 10, 2020·Scientific Reports·Nicholas S KruyerPamela Peralta-Yahya
Sep 27, 2020·Biotechnology Journal·Tobias SchwanemannBenedikt Wynands
Mar 14, 2021·Trends in Biotechnology·Allison L YaguchiMark A Blenner
Apr 1, 2021·Biotechnology Advances·Maria Martin-PascualVitor A P Martins Dos Santos
Apr 27, 2021·Frontiers in Microbiology·Tat-Ming LoMatthew Wook Chang
Aug 4, 2021·Nature Reviews. Microbiology·José Montaño LópezJosé L Avalos
Aug 14, 2020·ACS Synthetic Biology·Jack T SuitorStephen Wallace

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