Direct blood culturing on solid medium outperforms an automated continuously monitored broth-based blood culture system in terms of time to identification and susceptibility testing

New Microbes and New Infections
E A IdelevichK Becker

Abstract

Pathogen identification and antimicrobial susceptibility testing (AST) should be available as soon as possible for patients with bloodstream infections. We investigated whether a lysis-centrifugation (LC) blood culture (BC) method, combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) identification and Vitek 2 AST, provides a time advantage in comparison with the currently used automated broth-based BC system. Seven bacterial reference strains were added each to 10 mL human blood in final concentrations of 100, 10 and 1 CFU/mL. Inoculated blood was added to the Isolator 10 tube and centrifuged at 3000 g for 30 min, then 1.5 mL sediment was distributed onto five 150-mm agar plates. Growth was observed hourly and microcolonies were subjected to MALDI-TOF MS and Vitek 2 as soon as possible. For comparison, seeded blood was introduced into an aerobic BC bottle and incubated in the BACTEC 9240 automated BC system. For all species/concentration combinations except one, successful identification and Vitek 2 inoculation were achieved even before growth detection by BACTEC. The fastest identification and inoculation for AST were achieved with Escherichia coli in concentrations of 100 ...Continue Reading

References

Dec 12, 2012·Archives of Pathology & Laboratory Medicine·Katherine K PerezJames M Musser
Dec 25, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Olivier ClercGilbert Greub
Aug 1, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Angela M HuangJerod L Nagel
Apr 5, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E A IdelevichK Becker
Sep 26, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A VerrokenY Glupczynski
Mar 11, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·O OpotaG Greub
May 6, 2015·Journal of Microbiological Methods·Jean-Benoît ZabbeEmilie Bessède

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Citations

Apr 15, 2016·International Journal of Molecular Sciences·Maria Teresa GalloFabrizio Ensoli
Oct 30, 2016·Expert Review of Molecular Diagnostics·Teresa CimminoJean-Marc Rolain
Nov 15, 2018·Future Microbiology·Joseph M Blondeau, Evgeny A Idelevich
Jan 27, 2019·Deutsches Ärzteblatt International·Evgeny A IdelevichKarsten Becker

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Methods Mentioned

BETA
imaging technology

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