Direct blood dry LAMP: a rapid, stable, and easy diagnostic tool for Human African Trypanosomiasis

PLoS Neglected Tropical Diseases
Kyoko HayashidaChihiro Sugimoto

Abstract

Loop-mediated isothermal amplification (LAMP) is a rapid and sensitive tool used for the diagnosis of a variety of infectious diseases. One of the advantages of this method over the polymerase chain reaction is that DNA amplification occurs at a constant temperature, usually between 60-65°C; therefore, expensive devices are unnecessary for this step. However, LAMP still requires complicated sample preparation steps and a well-equipped laboratory to produce reliable and reproducible results, which limits its use in resource-poor laboratories in most developing countries. In this study, we made several substantial modifications to the technique to carry out on-site diagnosis of Human African Trypanosomiasis (HAT) in remote areas using LAMP. The first essential improvement was that LAMP reagents were dried and stabilized in a single tube by incorporating trehalose as a cryoprotectant to prolong shelf life at ambient temperature. The second technical improvement was achieved by simplifying the sample preparation step so that DNA or RNA could be amplified directly from detergent-lysed blood samples. With these modifications, diagnosis of HAT in local clinics or villages in endemic areas becomes a reality, which could greatly impact ...Continue Reading

References

Jan 1, 1979·Transactions of the Royal Society of Tropical Medicine and Hygiene·W H LumsdenS J Doig
Jul 1, 1982·Molecular and Biochemical Parasitology·P BorstJ James
Apr 1, 1994·Tropical Doctor·J W Bailey, D H Smith
Mar 1, 1997·Tropical Medicine & International Health : TM & IH·H HirumiN Suzuki
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Jul 20, 2002·The Lancet Infectious Diseases·Dominique LegrosPhilippe Büscher
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Wendy GibsonAndrew Griffiths
Jan 18, 2005·Clinical Microbiology Reviews·François ChappuisPhilippe Büscher
Feb 7, 2008·PLoS Neglected Tropical Diseases·Zablon Kithinji NjiruAndrew Richard Christopher Thompson
Sep 17, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Naozumi TeramotoMitsuhiro Shibata
Apr 28, 2009·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yasuyoshi Mori, Tsugunori Notomi
Mar 20, 2010·Journal of Microbiological Methods·Motoki GotoKen-Ichi Hanaki
Feb 25, 2011·PLoS Neglected Tropical Diseases·Oliver BalmerAdalgisa Caccone
Mar 30, 2013·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yasuyoshi MoriTsugunori Notomi

❮ Previous
Next ❯

Citations

Nov 19, 2015·Parasites & Vectors·Chaichontat SriworaratPadet Siriyasatien
Oct 1, 2015·Parasites & Vectors·Dusit LaohasinnarongBoniface Namangala
Nov 5, 2016·Analytical Chemistry·Rhett L MartineauDeirdre R Meldrum
Jun 6, 2017·Expert Review of Molecular Diagnostics·Julio Alonso-PadillaJoaquim Gascon
Dec 13, 2016·PLoS Neglected Tropical Diseases·Isabel M VincentMichael P Barrett
Nov 2, 2016·Lab on a Chip·C S BallR J Meagher
Jul 17, 2015·Journal of Clinical Microbiology·Brianna L PetroneJonas M Winchell
Sep 21, 2019·Lab on a Chip·Elizabeth A PhillipsJacqueline C Linnes
Jun 11, 2020·Diagnostics·Emma Michelle Taylor
Oct 5, 2019·Journal of Human Genetics·Arthur Elia MonganLucky Ronald Runtuwene
Oct 5, 2020·Tropical Animal Health and Production·Thi Ngan MaiSatoshi Sekiguchi
May 13, 2020·Journal of Parasitology Research·Maxwell W WaemaHelena A Ngowi
May 20, 2016·PLoS Neglected Tropical Diseases·David SquarreBoniface Namangala
Jun 16, 2017·Scientific Reports·Junya YamagishiYutaka Suzuki
Oct 3, 2019·Biosensors·Faye M Walker, Kuangwen Hsieh
Aug 9, 2020·Lab on a Chip·Nikunja KolluriCatherine M Klapperich
Oct 18, 2018·The Onderstepoort Journal of Veterinary Research·Peter NambalaChihiro Sugimoto
Oct 23, 2020·PLoS Neglected Tropical Diseases·Kyoko HayashidaJunya Yamagishi
Jan 26, 2021·PLoS Neglected Tropical Diseases·Thoko Flav KapalamulaChie Nakajima
Jan 20, 2021·Analytica Chimica Acta·Micaela L EverittIan M White
Mar 3, 2021·PLoS Neglected Tropical Diseases·Joshua LongbottomMichelle C Stanton

❮ Previous
Next ❯

Datasets Mentioned

BETA
AJ345057
AJ345058

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

LAMP
clustalW

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.