PMID: 9636363Jun 24, 1998Paper

Direct cleavage of elF4G by poliovirus 2A protease is inefficient in vitro

Virology
M L BoveeR E Lloyd

Abstract

Previously, the purified recombinant 2A proteases (2Apro) of coxsackievirus B4 (CVB4) and human rhinovirus type 2 (HRV2) were shown to cleave synthetic peptides derived from human or rabbit elF4G as well as elF4G protein purified from rabbit reticulocytes. These results were in contrast to previous evidence which supported the view that elF4G cleavage activity in poliovirus-infected HeLa cells required a cellular factor(s) activated by poliovirus (PV) 2Apro. In the present study, recombinant PV 2Apro was shown to cleave either rabbit or human elF4G or their derived peptides in direct cleavage reactions, but cleaved the 4G-derived peptides with 100-fold lower efficiency than with a peptide derived from the poliovirus polyprotein. In these experiments, up to 25-fold molar excess of 2Apro over elF4G protein was required to cause greater than 50% cleavage. CVB4 2Apro was also tested in peptide cleavage assays under the same conditions as PV 2Apro and was found to cleave all elF4G substrates with efficiencies similar to PV 2Apro. Finally, cleavage reactions utilizing recombinant elF4G containing a G486E substitution at the cleavage site for CVB4 and HRV2 proteases resulted in drastically reduced cleavage by PV 2Apro, similar to the ...Continue Reading

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Citations

May 5, 2006·Applied and Environmental Microbiology·Yu-Chen HwangMarylynn V Yates
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·D S Lyles
Jun 29, 2000·Annual Review of Biochemistry·A C GingrasN Sonenberg
May 5, 2011·Journal of Biomedicine & Biotechnology·Alfredo CastellóLuis Carrasco
Feb 18, 2010·PloS One·Bryan K ClarksonJennifer A Doudna
Jan 8, 2013·Future Virology·Amanda J Chase, Bert L Semler
Apr 23, 2008·The Journal of Cell Biology·Francisco Ramírez-ValleRobert J Schneider
Nov 24, 2005·Virus Research·Richard E Lloyd
May 25, 2002·The Journal of General Virology·Rei-Lin KuoWu-Tse Liu
Nov 17, 2007·Cell Host & Microbe·James P WhiteRichard E Lloyd
Sep 14, 2002·The Journal of Biological Chemistry·Nicole FoegerTim Skern
Dec 16, 1998·Journal of Virology·I VentosoL Carrasco

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