PMID: 18421178Apr 19, 2008Paper

Direct comparison between ICSI-mediated DNA transfer and pronuclear DNA microinjection for producing transgenic rats

Experimental Animals
Masumi HirabayashiShinichi Hochi

Abstract

Production efficiency of transgenic rats was compared directly between the routine pronuclear microinjection of exogenous DNA solution (PNMI-Tg method) and the ooplasmic injection of sperm cells exposed to exogenous DNA solution (ICSI-Tg method) using six DNA constructs. The overall production efficiency per treated oocyte in the ICSI-Tg method (mean 1.1%, range 0.2 to 3.1%) was similar to that in the PNMI-Tg method (mean 1.1%, range 0 to 2.4%). An advantage of the ICSI-Tg method in the production of transgenic rats is noted in cases in which a low yield of pronuclear zygotes is an inevitable fate of the rat strain.

References

Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R L BrinsterR D Palmiter
May 15, 1999·Science·A C PerryR Yanagimachi
Feb 12, 2000·Transgenic Research·R TakahashiM Ueda
Jul 30, 2003·Transgenic Research·Elizabeth A MagaJames D Murray
Aug 5, 2004·Molecular Reproduction and Development·Megumi KatoMasumi Hirabayashi
May 3, 2006·Experimental Animals·Masumi HirabayashiShinichi Hochi

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Citations

Mar 6, 2009·Cellular and Molecular Neurobiology·Ondrej BugosNorbert Zilka
May 1, 2008·The Journal of Reproduction and Development·Masumi Hirabayashi
Mar 15, 2014·Nature Protocols·Zoltán IvicsZsuzsanna Izsvák

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