Direct comparison of capillary electrophoresis and capillary liquid chromatography hyphenated to collision-cell inductively coupled plasma mass spectrometry for the investigation of Cd-, Cu- and Zn-containing metalloproteins

Journal of Chromatography. a
María Montes-BayónAndreas Prange

Abstract

Capillary liquid chromatography (cLC) and capillary electrophoresis (CE) have been critically compared for the separation of metalloproteins when using collision-cell inductively coupled plasma mass spectrometry (ICP-CC-MS) as detection system. For cLC separation, the selected column was a C8 (0.3 mm I.D.) and the separation conditions involved a gradient up to 80% methanol in 10mM ammonium acetate buffer (pH 7.4). The low flow rate used (3 microL min(-1)) permitted the utilization of a high methanol content maintaining the sensitivity along the whole chromatographic run. For this purpose, a new low-flow interface has been developed based on a total consumption nebulizer. Similarly, CE has been studied as separation technique using a 75 microm I.D. fused silica capillary and a running buffer of 20 mM Tris-HNO3 (pH 7.4) and working at 30 kV. Metallothionein (mixture of MT-I and -II) and superoxide dismutase (SOD) have been used as protein models in order to evaluate the separation/detection capabilities using the same injection volumes in both systems (20 nL). For both hybrid systems, separation parameters such as retention factor, numbers of theoretical plates, tailing factor and resolution have been critically compared. Also, ...Continue Reading

References

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Citations

Feb 9, 2008·Analytical and Bioanalytical Chemistry·Takamitsu MiyayamaKazuo T Suzuki
Jun 29, 2010·Analytical and Bioanalytical Chemistry·Colin D MedleyCharles Demarest
Apr 7, 2012·Analytical and Bioanalytical Chemistry·Björn Meermann, Michael Sperling
Jul 8, 2010·Biological Trace Element Research·Fatemeh Shariati, Shahab Shariati
Sep 27, 2014·Nanoscale Research Letters·Hadi M MarwaniAbdullah M Asiri
Aug 30, 2008·Expert Opinion on Drug Metabolism & Toxicology·Bente GammelgaardCharlotte Møller
Nov 1, 2011·Journal of Chromatography. a·Marketa RyvolovaRene Kizek
Jun 15, 2007·Journal of Chromatography. a·Rob HaselbergGovert W Somsen
May 14, 2016·Environmental Monitoring and Assessment·Mohamed Abdulraheem ShaheenAbdulmohsin Rajeh Al-Shareef
Jul 14, 2017·Metallomics : Integrated Biometal Science·Matthew A GibsonJürgen Gailer
Jan 3, 2007·Journal of Mass Spectrometry : JMS

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