Direct Comparison of Standard Transmission Electron Microscopy and Cryogenic-TEM in Imaging Nanocrystals Inside Liposomes

Molecular Pharmaceutics
Tang LiBen J Boyd

Abstract

The use of electron microscopy techniques in the understanding of shape and size of nanoparticles are commonly applied to drug nanotechnology, but the type of microscopy and suitability for the particles of interest can have a significant impact on the result. The size and shape of the nanoparticles are crucial in clinical applications; however, direct comparison of the results from standard transmission electron microscopy (TEM) and cryo-TEM have rarely been reported. As a useful case for comparison, liposomal drug nanocrystals are studied here. In this study, the effect of thawing temperature on the size and shape of the ciprofloxacin nanocrystals was determined. A quantitative standard TEM assay was developed to allow for high-throughput particle size analysis. These results were compared to size and shape information obtained using the cryo-TEM method. The results showed broad agreement between the two TEM methods and that ciprofloxacin nanocrystals formed shorter and thinner crystals inside the liposomes at higher thawing temperatures. The results provide confidence in the use of standard TEM to determine the size and shape distribution of solid nanoparticles (in this case, encapsulated inside liposomes) from aqueous media...Continue Reading

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Citations

Mar 3, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·M GhezziS Nicoli
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fakhara SabirIldikó Csóka
Jul 31, 2021·Chemical Reviews·Aoon RizviJoseph P Patterson
Jul 30, 2021·International Journal of Pharmaceutics·Fernando Freitas de LimaEneida de Paula

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