Direct Comparison of the Enzymatic Characteristics and Superoxide Production of the Four Aldehyde Oxidase Enzymes Present in Mouse

Drug Metabolism and Disposition : the Biological Fate of Chemicals
Gökhan KücükgözeSilke Leimkühler

Abstract

Aldehyde oxidases (AOXs) are molybdoflavoenzymes with an important role in the metabolism and detoxification of heterocyclic compounds and aliphatic as well as aromatic aldehydes. The enzymes use oxygen as the terminal electron acceptor and produce reduced oxygen species during turnover. Four different enzymes, mAOX1, mAOX3, mAOX4, and mAOX2, which are the products of distinct genes, are present in the mouse. A direct and simultaneous comparison of the enzymatic properties and characteristics of the four enzymes has never been performed. In this report, the four catalytically active mAOX enzymes were purified after heterologous expression inEscherichia coliThe kinetic parameters of the four mouse AOX enzymes were determined and compared with the use of six predicted substrates of physiologic and toxicological interest, i.e., retinaldehyde,N1-methylnicotinamide, pyridoxal, vanillin, 4-(dimethylamino)cinnamaldehyde (p-DMAC), and salicylaldehyde. While retinaldehyde, vanillin,p-DMAC, and salycilaldehyde are efficient substrates for the four mouse AOX enzymes,N1-methylnicotinamide is not a substrate of mAOX1 or mAOX4, and pyridoxal is not metabolized by any of the purified enzymes. Overall, mAOX1, mAOX2, mAOX3, and mAOX4 are charac...Continue Reading

References

Sep 15, 1992·Biochimica Et Biophysica Acta·R Pérez-VicenteM Pineda
Sep 15, 1974·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K H Wurzinger, R Hartenstein
Jul 1, 1971·Archives of Biochemistry and Biophysics·M Stanulović, S Chaykin
Jun 1, 1972·Archives of Biochemistry and Biophysics·T A KrenitskyG H Hitchings
Jan 8, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·R Scott Obach
Mar 14, 2007·Archives of Biochemistry and Biophysics·Tapan Kumar KunduJay L Zweier
Apr 19, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Luisa MaiaLurdes Mira
Dec 11, 2007·Cellular and Molecular Life Sciences : CMLS·E GarattiniM Terao
Mar 12, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Shigeyuki KitamuraShigeru Ohta
Dec 30, 2009·Human Genomics·Enrico GarattiniMineko Terao
Sep 22, 2010·Journal of Medicinal Chemistry·David C PrydeThien-Duc Tran
Mar 25, 2011·Drug Metabolism Reviews·Enrico Garattini, Mineko Terao
Jun 28, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Martin MahroSilke Leimkühler
Jan 27, 2012·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Tobias HartmannSilke Leimkühler
Feb 18, 2012·Expert Opinion on Drug Metabolism & Toxicology·Enrico Garattini, Mineko Terao
Sep 29, 2012·The Journal of Biological Chemistry·Catarina CoelhoMaria João Romão
Nov 3, 2012·Current Pharmaceutical Design·Ken OkamotoTakeshi Nishino
Dec 18, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Shigeyuki Kitamura, Kazumi Sugihara
Jul 2, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ralf R Mendel, Silke Leimkühler
Oct 8, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Nuno M F S A CerqueiraMaria João Ramos
Sep 1, 2015·Nature Chemical Biology·Catarina CoelhoMaria João Romão
Feb 5, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Alessandro FotiSilke Leimkühler
Feb 28, 2016·Archives of Toxicology·Mineko TeraoEnrico Garattini

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Citations

Jan 24, 2019·Journal of Inherited Metabolic Disease·Matthew P WilsonPeter T Clayton
Mar 8, 2020·The Journal of Biological Chemistry·Mineko TeraoSilke Leimkühler
Jun 1, 2019·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Christine Beedham
Jan 5, 2019·Molecular Brain·Mahmoudreza RaminYong Rao
Nov 25, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Jolita CiapaiteNanda M Verhoeven-Duif
Jan 30, 2021·The Journal of Biological Chemistry·Mineko TeraoSilke Leimkühler

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