PMID: 6413637Oct 1, 1983Paper

Direct demonstration of the identity of T cell growth factor binding protein and the Tac antigen

The Journal of Experimental Medicine
R J Robb, W C Greene

Abstract

Radiolabeled molecules from detergent-solubilized human T cell blasts were fractionated on affinity supports coupled with T cell growth factor (TCGF) and anti-Tac antibody. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that a glycoprotein of approximately 58,000 mol wt was bound in both cases. Sequential binding to the two affinity supports demonstrated that the molecules recognized in each instance were identical. Thus, the Tac antigen contains the cellular binding site for TCGF. Note added in proof: Preliminary binding experiments using high concentrations of [3H]leucine, lysine TCGF with a low specific radioactivity indicate the existence of a sizeable pool of receptor sites with an affinity 2,000-10,000 times lower than that of the high affinity receptors measured in Fig. 1. Such sites may explain the numerical discrepancy between early TCGF binding experiments (5) and the binding of the anti-Tac antibody. The hypothesis that the anti-Tac antibody apparently reacts with both classes of receptor would explain its effect on the physiological response (high affinity binding) and the high level of Tac antigen on the cell surface.

Citations

Sep 1, 1990·Arthritis and Rheumatism·K S BarronF M Orson
Mar 1, 1985·Journal of Clinical Immunology·H Tsuda, K Takatsuki
Jul 1, 1987·Journal of Clinical Immunology·T MiyawakiM D Cooper
Sep 1, 1995·Medical Oncology·A TorenA Nagler
Jan 1, 1994·Biotherapy·M KatanoJ Tadano
Oct 26, 1984·Journal of Immunological Methods·K SugamuraY Hinuma
Apr 21, 1989·Journal of Immunological Methods·A Harel-BellanW L Farrar
Aug 29, 1996·Mechanisms of Ageing and Development·M A Pahlavani, A Richardson
Jan 1, 1991·Developmental and Comparative Immunology·T H Lee, C H Tempelis
Jun 24, 1987·Biochimica Et Biophysica Acta·W Curatolo
Dec 16, 1992·Biochimica Et Biophysica Acta·Y MinamiT Taniguchi
Jun 1, 1997·Immunology Letters·K StenroosC Lindqvist
Nov 5, 2002·Journal of Ethnopharmacology·Shu-Cheng WengYuh-Chi Kuo
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M KrönkeW C Greene
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D L UrdalS K Dower
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M E HemlerJ L Strominger
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J C ReedR G Hoover
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V DuprezA Dautry-Varsat
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A A YthierT B Strom
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K A Smith, D A Cantrell
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R J Robb
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D S TaylorJ Kornbluth
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M TsudoT A Waldmann
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A M WeissmanR D Klausner
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M E CosulichA Bargellesi
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S CampbellB Haley
Aug 1, 1984·The Journal of Experimental Medicine·M TsudoH Uchino
Nov 1, 1984·The Journal of Experimental Medicine·K WelteR Mertelsmann
Nov 1, 1984·The Journal of Experimental Medicine·T A WaldmannW C Greene
Jul 1, 1985·The Journal of Experimental Medicine·W C GreeneW J Leonard
May 1, 1986·The Journal of Experimental Medicine·Z ToossiJ J Ellner
Nov 6, 1989·European Journal of Biochemistry·J P PiauG Gacon
Mar 1, 1993·The American Review of Respiratory Disease·A PforteH W Ziegler-Heitbrock
Jul 1, 1992·American Journal of Respiratory Cell and Molecular Biology·G H DownieM Friedman

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