Direct Detection and Identification of Prosthetic Joint Infection Pathogens in Synovial Fluid by Metagenomic Shotgun Sequencing

Journal of Clinical Microbiology
Morgan I IvyRobin Patel

Abstract

Metagenomic shotgun sequencing has the potential to transform how serious infections are diagnosed by offering universal, culture-free pathogen detection. This may be especially advantageous for microbial diagnosis of prosthetic joint infection (PJI) by synovial fluid analysis since synovial fluid cultures are not universally positive and since synovial fluid is easily obtained preoperatively. We applied a metagenomics-based approach to synovial fluid in an attempt to detect microorganisms in 168 failed total knee arthroplasties. Genus- and species-level analyses of metagenomic sequencing yielded the known pathogen in 74 (90%) and 68 (83%) of the 82 culture-positive PJIs analyzed, respectively, with testing of two (2%) and three (4%) samples, respectively, yielding additional pathogens not detected by culture. For the 25 culture-negative PJIs tested, genus- and species-level analyses yielded 19 (76%) and 21 (84%) samples with insignificant findings, respectively, and 6 (24%) and 4 (16%) with potential pathogens detected, respectively. Genus- and species-level analyses of the 60 culture-negative aseptic failure cases yielded 53 (88%) and 56 (93%) cases with insignificant findings and 7 (12%) and 4 (7%) with potential clinically ...Continue Reading

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References

Jan 19, 2010·Bioinformatics·Heng Li, Richard Durbin
Oct 12, 2010·The Journal of Antimicrobial Chemotherapy·E MoranB L Atkins
Nov 15, 2011·The Journal of Arthroplasty·UNKNOWN Workgroup Convened by the Musculoskeletal Infection Society
Oct 3, 2012·PLoS Pathogens·Gilda GrardEric M Leroy
Nov 10, 2012·The International Journal of Artificial Organs·Stéphane CorvecAndrej Trampuz
Dec 12, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Douglas R OsmonUNKNOWN Infectious Diseases Society of America
Jul 6, 2013·Bioinformatics·Sasha K AmesJonathan E Allen
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Apr 4, 2014·Clinical Microbiology Reviews·Aaron J Tande, Robin Patel
Jun 5, 2014·The New England Journal of Medicine·Michael R WilsonCharles Y Chiu
Jan 13, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Samia N NaccacheCharles Y Chiu
Jul 15, 2015·The New England Journal of Medicine·Bernd HoffmannMartin Beer
Sep 12, 2015·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yuto FukuiKazuhiro Tateda
Sep 30, 2015·Nature Methods·Duy Tin TruongNicola Segata
Dec 8, 2015·Journal of Clinical Microbiology·Yanjiao ZhouGeorge M Weinstock
May 31, 2016·Journal of Microbiological Methods·Matthew ThoendelRobin Patel
Jun 25, 2016·Neurology. Neuroimmunology and Neuroinflammation·Steven L SalzbergCarlos A Pardo
Jan 8, 2017·Journal of the Pediatric Infectious Diseases Society·Kanokporn MongkolrattanothaiCharles Y Chiu
Apr 6, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthew ThoendelRobin Patel
May 6, 2017·American Journal of Respiratory and Critical Care Medicine·Kathryn M PendletonRobert P Dickson
May 10, 2017·Open Forum Infectious Diseases·Nguyen Thi Hoang MaiLe Van Tan
Jun 20, 2017·Annals of Neurology·Michael R WilsonJoseph L DeRisi
Jul 8, 2017·American Journal of Respiratory and Critical Care Medicine·Charles LangelierJoseph L DeRisi
Aug 12, 2017·Scientific Reports·Etienne RuppéJacques Schrenzel
Oct 19, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Patricia J SimnerKaren C Carroll
Jan 18, 2018·The Journal of Bone and Joint Surgery. American Volume·Majd TarabichiJavad Parvizi
Apr 13, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthew J ThoendelRobin Patel

❮ Previous
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Citations

Jul 7, 2018·Journal of Clinical Microbiology·John P Dekker
Nov 6, 2018·The Journal of Rheumatology·Yvonne Achermann, Annelies S Zinkernagel
Mar 29, 2019·Nature Reviews. Genetics·Charles Y Chiu, Steven A Miller
May 29, 2019·Journal of Medical Microbiology·Chao QiRichard G Wunderink
Aug 25, 2020·Annals of Laboratory Medicine·Yiming ZhongMarilyn M Li
Sep 21, 2019·The Journal of Infectious Diseases·Augusto Dulanto Chiang, John P Dekker
Jan 23, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Benjamin F RicciardiEdward M Schwarz
Nov 23, 2019·EFORT Open Reviews·Jeya PalanPedro Foguet
Feb 8, 2020·Current Infectious Disease Reports·Erin H Graf, Preeti Pancholi
Oct 13, 2020·Frontiers in Cellular and Infection Microbiology·Zi-da HuangJian-Hua Lin
Nov 27, 2020·Frontiers in Medicine·Rossella Baldan, Parham Sendi
Jan 29, 2021·International Journal of Environmental Research and Public Health·Giorgia CarusoTeresa Fasciana
Oct 1, 2019·Journal of Orthopaedic Trauma·Melissa DepypereUNKNOWN Fracture-Related Infection (FRI) Consensus Group
Apr 9, 2021·EFORT Open Reviews·Jaime Esteban, Enrique Gómez-Barrena
May 28, 2021·EFORT Open Reviews·Pier F IndelliDerek F Amanatullah
Mar 2, 2021·Expert Review of Molecular Diagnostics·Zakareya GamieEleftherios Tsiridis
Jul 6, 2021·Diagnostic Microbiology and Infectious Disease·Laure FlurinRobin Patel
Jul 30, 2021·Open Forum Infectious Diseases·Nanda RamchandarChristopher Cannavino
Jul 15, 2021·Journal of Paediatrics and Child Health·Pablo Yagupsky
Oct 22, 2021·Frontiers in Cellular and Infection Microbiology·Chunrong HuangYun Feng
Jul 4, 2020·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T N Peel, R de Steiger
Nov 16, 2021·Microbial Genomics·Tommi MäklinAntti Honkela
Feb 6, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jason Scott LipofIrvin Oh
Dec 25, 2021·Journal of the Pediatric Infectious Diseases Society·Priya Edward, Andrew S Handel

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