Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL.

Journal of Clinical Microbiology
D De VosP Cornelis

Abstract

A multiplex PCR test based on the simultaneous amplification of two lipoprotein genes, oprI and oprL, was designed and evaluated for its ability to directly detect fluorescent pseudomonads (amplification of oprI open reading frame, 249 bp) and Pseudomonas aeruginosa (amplification of oprL open reading frame, 504 bp) in clinical material. A collection of reference strains including 20 different species of fluorescent pseudomonads was tested. Positive PCR results for both genes were observed only for P. aeruginosa isolates (n = 150), including strains of clinical and environmental origin, while only one gene, oprI, was amplified from the other fluorescent pseudomonads. All other bacteria tested (n = 15) were negative by the amplification test. The lower detection level for P. aeruginosa was estimated to be 10(2) cells/ml. Preliminary evaluation on testing skin biopsy specimens from patients with burns (n = 14) and sputum samples from cystic fibrosis patients (n = 49) and other patients (n = 19) showed 100% sensitivity and 74% specificity in comparison with culture. This multiplex PCR assay appears promising for the rapid and sensitive detection of P. aeruginosa in clinical specimens. Further evaluation of its specificity in longi...Continue Reading

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Citations

Oct 6, 2006·FEMS Microbiology Letters·Sarah DenayerPierre Cornelis
Nov 26, 2009·PloS One·Jean-Paul PirnayDaniel De Vos
Feb 8, 2011·American Journal of Respiratory and Critical Care Medicine·Eilidh MowatCraig Winstanley
Oct 30, 2012·Journal of Medical Microbiology·Amanda J HallCraig Winstanley
Mar 19, 2013·Applied Microbiology and Biotechnology·Hyeon Jin ChoiDong Suk Park
Dec 15, 2015·BMC Microbiology·Hend ZeitounNourhan Fanaki
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Jun 11, 2005·Environmental Microbiology·Jean-Paul PirnayPierre Cornelis
May 13, 2005·Journal of Medical Microbiology·Craig WinstanleyUNKNOWN Microbiology Ophthalmic Group
Mar 31, 2015·International Journal of Immunopathology and Pharmacology·Abdolaziz Rastegar LariMorovat Taherikalani
Jul 9, 2014·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Rui SeixasManuela Oliveira
Jul 15, 2016·Journal of Clinical Microbiology·Saara SillanpääOndrej Cinek
Jan 25, 2020·FEMS Microbiology Letters·Edward Cunningham-OakesEshwar Mahenthiralingam
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Jan 22, 2003·Environmental Microbiology·Jean-Paul PirnayPierre Cornelis
Apr 11, 2003·Microbiology·Magaly de ChialPierre Cornelis

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