Direct detection of endogenous MicroRNAs and their post-transcriptional modifications in cancer serum by capillary electrophoresis-mass spectrometry

Analytical and Bioanalytical Chemistry
Nasrin KhanMaxim V Berezovski

Abstract

MicroRNA molecules (miRNAs) are a class of small, single-stranded, non-coding RNA molecules that regulate cellular messenger RNA and their corresponding proteins. Extracellular miRNAs circulate in the bloodstream inside exosomes or in complexes with proteins and lipoproteins. The miRNA sequences and their quantitative levels are used as unique signatures associated with cancer diagnosis and prognosis after anticancer treatment. MicroRNAs are modified through a series of processing events after transcription like 5'-end phosphorylation, 3'- end adenylation or uridylation, terminal nucleotide deletion. The problem is that existing bioanalytical methods such as microarrays and a quantitative polymerase chain reaction are sensitive, but not capable of identifying the post-transcriptional modifications of miRNA. Here we report a capillary electrophoresis-mass spectrometry (CE-MS) method, which performs a multiplex, direct analysis of miRNAs from biological samples. Using the CE-MS method, we detected two endogenous human circulating miRNAs, a 23-nucleotide long 5'-phosporylated miRNA with 3'-uridylation (iso-miR-16-5p), and a 22-nucleotide long 5'-phosporylated miRNA (miR-21-5p) isolated from B-cell chronic lymphocytic leukemia seru...Continue Reading

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Citations

Mar 9, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gaia PalminiMaria Luisa Brandi
Oct 27, 2016·Electrophoresis·Anna TýčováKarel Klepárník
Oct 17, 2017·Analytical and Bioanalytical Chemistry·Despina P KalogianniTheodore K Christopoulos
Feb 3, 2019·Analytical and Bioanalytical Chemistry·Roberta D'Agata, Giuseppe Spoto
Jun 9, 2020·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Diem Hong Tran, Huong Thi Thu Phung
Dec 26, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kellen DeLaneyLingjun Li
Jul 2, 2017·Cancer Metastasis Reviews·Preeti Kanikarla-MarieScott Kopetz
Jan 7, 2020·Mass Spectrometry Reviews·Jia-Jia CuiJi-Ye Yin
Sep 14, 2018·Analytical Chemistry·Bei ChenYu-Qi Feng
Jan 4, 2018·Analytical Chemistry·Robert L C VoetenGovert W Somsen

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