Direct detection of Kitasatospora species with a chaperone oligonucleotide microarray method lacking PCR amplification

Journal of Basic Microbiology
Sebastian GuentherThomas Munder

Abstract

Identification of members of the genus Kitasatospora from soil samples has been introduced to evaluate occurrence of potential natural compound producers in different habitats. The microarray hybridization usually involves PCR amplification of the target DNA. Since PCR might lead to biased amplification, a diagnostic Kitasatospora microarray technique was improved by a protocol lacking PCR amplification prior to hybridization. The described advanced hybridization method used chaperone oligonucleotides for direct co-hybridization with genomic DNA on an oligonuclotide microarray with optical readout.

References

Oct 23, 1997·International Journal of Systematic Bacteriology·Z ZhangJ Ruan
Apr 17, 1999·Microbiology·V Gürtler, B C Mayall
Jun 20, 2001·International Journal of Systematic and Evolutionary Microbiology·A S Anderson, E M Wellington
Sep 26, 2001·Applied and Environmental Microbiology·J SmallD P Chandler
Aug 16, 2005·Journal of Microbiological Methods·Sebastian GüntherThomas Munder
Nov 13, 2007·Applied Microbiology and Biotechnology·Robert MöllerWolfgang Fritzsche

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Citations

Apr 21, 2009·Analytical and Bioanalytical Chemistry·Jan Weile, Cornelius Knabbe
Feb 11, 2010·Future Microbiology·Tomasz A LeskiBaochuan Lin

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