Direct determination of verapamil in urine and serum samples by micellar liquid chromatography and fluorescence detection

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Maria Rambla-AlegreJ Esteve-Romero

Abstract

Verapamil, a calcium channel antagonist, is one of the most commonly prescribed drugs in the treatment of hypertension. In this work, it was determined in serum and urine samples by a sensitive and precise chromatographic procedure without any pre-treatment step in a C18 column using a micellar mobile phase of 0.15M sodium dodecyl sulfate and 5% pentanol at pH 7. Fluorescence detection set at 230 nm (excitation) and 312 nm (emission) was used. Verapamil is eluted at 12.5 min with no interference by the protein band or endogenous compounds. Linearities (r > 0.998), as well as intra- and inter-day precision, were studied in the validation of the method. LODs were also calculated to be 11.0, 18.5 and 20.2 ng/mL in micellar solution, serum and urine, respectively. Recoveries in the biological matrices were in the 97-99% range. Drug excretion in urine was studied in a volunteer receiving treatment for hypertension, and verapamil, as an unchanged drug, was separated from other metabolites. The procedure developed can be useful in the field of toxicology and clinical analysis.

References

May 14, 2003·Forensic Science International : Synergy·Hiroshi KinoshitaShigeru Hishida
Jun 3, 2004·Forensic Science International : Synergy·F MusshoffB Madea

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Citations

Apr 28, 2010·Analytical and Bioanalytical Chemistry·Josep Esteve-RomeroAdrià Martinavarro-Domínguez
Nov 19, 2010·Bioanalysis·María Jose Ruiz-ÁngelMaria Celia García-Álvarez-Coque
Oct 27, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maria Rambla-AlegreSamuel Carda-Broch
Dec 30, 2014·Journal of Chromatographic Science·Hassan M AlbishriRoaa A Tayeb
Nov 9, 2011·The Analyst·Beatriz Beltrán-MartinavarroSamuel Carda-Broch

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