PMID: 11483217Aug 3, 2001Paper

Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction

Journal of Virological Methods
A VabretF Freymuth

Abstract

An RT-PCR-hybridization was developed that amplified genetic material from the M protein gene of HCoV-229E and HCoV-OC43. The analytic sensitivity of these original primers were compared with primers defined in the N gene and described previously. The results show that 0.05 TCID50 of HCoV-229E and 0.01 TCID50 of HCoV-OC43 can be detected by this molecular method using the original method. Detection of HCoV-229E and HCoV-OC43 in clinical specimens is possible using this method: 348 respiratory specimens (202 sputum and 146 nasal aspirates) were tested with this RT-PCR-hybridization and 12 human coronavirus are detected (3%). The method could provide a useful tool for demonstrating the role of human coronavirus in infections of the respiratory tract.

References

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Citations

Apr 25, 2003·Lancet·J S M PeirisUNKNOWN SARS study group
May 22, 2003·Paediatric Respiratory Reviews·P L Mackie
Apr 10, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Astrid VabretFrançois Freymuth
Sep 18, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Frédéric PenePierre Lebon
Nov 13, 2009·The Pediatric Infectious Disease Journal·Lay Myint YoshidaKoya Ariyoshi
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A BerminghamM C Zambon
Nov 15, 2007·Journal of Paediatrics and Child Health·Astrid VabretFrançois Freymuth
Sep 3, 2010·Journal of Clinical Microbiology·Rong-Fang ZhangZhaojun Duan
Sep 7, 2007·Journal of Clinical Microbiology·W Y LamPaul K S Chan
Aug 1, 2008·Journal of Clinical Microbiology·Xin-hui YuanZhao-jun Duan
Dec 1, 2006·Journal of Clinical Microbiology·Baochuan LinDavid A Stenger
Oct 7, 2006·Critical Care : the Official Journal of the Critical Care Forum·Cédric DaubinPierre Charbonneau
Aug 17, 2005·Emerging Infectious Diseases·Astrid VabretFrançois Freymuth
Jul 7, 2005·Intensive Care Medicine·Cédric DaubinPierre Charbonneau
Apr 7, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Frank EsperYung T Huang
Oct 29, 2010·Journal of Medical Virology·Jiad N McheikGérard Agius
Dec 24, 2009·Journal of Medical Virology·Li-shu ZhengZhao-jun Duan
Jun 14, 2018·Journal of Medical Virology·Chantal J SnoeckClaude P Muller
Sep 29, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ehsan ShabaniMohammad J Abdekhodaie

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