PMID: 11483217Aug 3, 2001Paper

Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction

Journal of Virological Methods
A VabretF Freymuth


An RT-PCR-hybridization was developed that amplified genetic material from the M protein gene of HCoV-229E and HCoV-OC43. The analytic sensitivity of these original primers were compared with primers defined in the N gene and described previously. The results show that 0.05 TCID50 of HCoV-229E and 0.01 TCID50 of HCoV-OC43 can be detected by this molecular method using the original method. Detection of HCoV-229E and HCoV-OC43 in clinical specimens is possible using this method: 348 respiratory specimens (202 sputum and 146 nasal aspirates) were tested with this RT-PCR-hybridization and 12 human coronavirus are detected (3%). The method could provide a useful tool for demonstrating the role of human coronavirus in infections of the respiratory tract.


Jun 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C J TriggR J Davies
Aug 12, 1999·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·F FreymuthB Guillois
Feb 29, 2000·The Pediatric Infectious Disease Journal·J Nokso-KoivistoT Hovi

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