Direct DNA transfer to plant cells

Plant Molecular Biology
M R DaveyB J Mulligan

Abstract

A range of somatic cell and molecular techniques are now available to supplement conventional plant breeding. The introduction and expression of foreign DNA has been used to modify basic aspects of physiology and development, to introduce commercially important characteristics such as herbicide and insect resistance into plants and to insert genes suitable as dominant selectable markers for somatic hybridisation. Several techniques for direct DNA delivery are available, ranging from uptake of DNA into isolated protoplasts mediated by chemical procedures or electroporation, to injection and the use of high-velocity particles to introduce DNA into intact tissues. Direct DNA uptake is applicable to both stable and transient gene expression studies and utilises a range of vectors, including those employed for gene cloning. Although the frequency of stable transformation is low, direct DNA uptake is applicable to those plants not amenable to Agrobacterium transformation, particularly monocotyledons.

References

May 1, 1989·Molecular & General Genetics : MGG·B DammL Willmitzer
Jan 1, 1989·The Plant Cell·R TöpferH H Steinbiss
Apr 8, 1988·Science·C A RhodesJ J Detmer
Jan 1, 1985·Molecular & General Genetics : MGG·I PotrykusR D Shillito
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M FrommV Walbot
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J R Ecker, R W Davis
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C KonczA A Szalay
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P ChristouW F Swain
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T M KleinP Maliga
Apr 1, 1987·Plant Physiology·R S BostonT K Hodges
Jul 1, 1988·Plant Physiology·P ChristouW F Swain
Dec 1, 1988·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W Zhang, R Wu
Dec 1, 1988·Plant Cell Reports·F BekkaouiD I Dunstan
Jul 1, 1987·Plant Cell Reports·F KöhlerO Schieder
Dec 1, 1985·Plant Cell Reports·W H LangridgeA A Szalay
Jan 1, 1987·Plant Molecular Biology·K Lindsey, M G Jones

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Citations

May 1, 1991·Plant Molecular Biology·B TinlandL Otten
Oct 20, 2005·Journal of Bioscience and Bioengineering·Takefumi SoneKiichi Fukui
Jan 9, 2007·Plant Biotechnology Journal·Stephen J Streatfield
Feb 8, 2005·Biotechnology Advances·Michael R DaveyKenneth C Lowe
Sep 26, 2006·Methods : a Companion to Methods in Enzymology·Luca SantiHugh Mason
Jun 19, 2012·Physics of Life Reviews·Ana Leonor RiveraAchim M Loske
Mar 1, 1992·Bio/technology·T M KleinS Fitzpatrick-McElligott
Aug 1, 1990·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D W James, H K Dooner

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