Direct effect of Ca2+-calmodulin on cGMP-activated Ca2+-dependent Cl-channels in rat mesenteric artery myocytes

The Journal of Physiology
A S Piper, W A Large

Abstract

Recently a novel cGMP-activated Ca2+-dependent Cl- channel has been described in rat mesenteric artery smooth muscle cells. In the present work we have investigated the actions of calmodulin (CaM) on single channel cGMP-activated Ca2+-dependent Cl- current (ICl(cGMP,Ca) in inside-out patches. When 1 microm CaM was applied to the intracellular surface of inside-out patches bathed with 10 microm cGMP and 100 nm [Ca2+]i there was approximately a 10-fold increase in channel open probability (NPo). This effect of CaM was not observed with lower [Ca2+]i and 100 nm [Ca2+]i with 1 microm CaM did not activate Cl- channels in the absence of cGMP. The unitary conductance, reversal potential and mean open time of the single-channel currents were similar in the absence or presence of CaM. With 10 microm cGMP and 100 nm [Ca2+]i the relationship between NPo and CaM concentration was well fitted by the Hill equation yielding an equilibrium constant for CaM of about 1.9 nm and a Hill coefficient of 1.7. With 1 microm CaM (+10 microm cGMP) the relationship between [Ca2+]i and NPo was also fitted by the Hill equation which yielded an apparent equilibrium constant of 74 nm [Ca2+]i and a Hill coefficient of 4.8. When [Ca2+]i was increased from 300 ...Continue Reading

References

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Citations

Mar 2, 2005·Basic Research in Cardiology·Benedetta TantiniNazzareno Galié
Apr 19, 2011·Cardiovascular Research·Torbjoern BroeggerVladimir V Matchkov
Oct 29, 2013·Pflügers Archiv : European journal of physiology·Vibeke Secher DamVladimir Matchkov
Nov 24, 2004·FEBS Letters·Valentin DutaMarek Duszyk
Nov 23, 2012·Journal of Vascular Research·Vladimir V MatchkovChristian Aalkjær
Aug 12, 2016·Physiology·Ebbe BoedtkjerChristian Aalkjaer
Aug 11, 2005·Canadian Journal of Physiology and Pharmacology·Normand LeblancIain A Greenwood
Apr 27, 2011·Acta Physiologica·C AalkjærV Matchkov
Feb 3, 2012·Physiological Reviews·J T SylvesterJeremy P T Ward
Jul 10, 2013·Molecular Medicine Reports·Katarzyna Szadujkis-SzadurskaMichal Wicinski
Jan 29, 2005·British Journal of Pharmacology·Christian Aalkjaer, Holger Nilsson
Jul 27, 2012·Journal of Cellular Physiology·Dulce Figueiras-FierroAlberto Darszon

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