Direct effect of curcumin on porcine ovarian cell functions

Animal Reproduction Science
A KadasiAlexander V Sirotkin

Abstract

Curcuma longa Linn (L.) is a plant widely used in cooking (in curry powder a.o.) and in folk medicine, but its action on reproductive processes and its possible mechanisms of action remain to be investigated. The objective of this study was to examine the direct effects of curcumin, the major Curcuma longa L. molecule, on basic ovarian cell functions such as proliferation, apoptosis, viability and steroidogenesis. Porcine ovarian granulosa cells were cultured with and without curcumin (at doses of 0, 1, 10 and 100μg/ml of medium). Markers of proliferation (accumulation of PCNA) and apoptosis (accumulation of bax) were analyzed by immunocytochemistry. The expression of mRNA for PCNA and bax was detected by RT-PCR. Cell viability was detected by trypan blue exclusion test. Release of steroid hormones (progesterone and testosterone) was measured by enzyme immunoassay (EIA). It was observed that addition of curcumin reduced ovarian cell proliferation (expression of both PCNA and its mRNA), promoted apoptosis (accumulation of both bax and its mRNA), reduced cell viability, and stimulated both progesterone and testosterone release. These observations demonstrate the direct suppressive effect of Curcuma longa L./curcumin on female gon...Continue Reading

Citations

Feb 27, 2018·Phytotherapy Research : PTR·Vahid SoleimaniHossein Hosseinzadeh
May 29, 2018·Oncotarget·Gloria M CalafFrancisco Aguayo
Sep 12, 2020·Environmental Science and Pollution Research International·Alexander V SirotkinAbdel Halim Harrath
May 23, 2020·Reproductive Toxicology·Beatriz Moreira-PintoIrene Rebelo
Aug 21, 2021·Planta medica·Alexander V Sirotkin
Nov 14, 2021·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Fatma Nur KarakusSeyhun Solakoglu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis