Direct effect of progestogen on gene expression in the testis during gonadotropin withdrawal and early suppression of spermatogenesis

The Journal of Clinical Endocrinology and Metabolism
Melanie J WaltonRichard A Anderson

Abstract

Testicular production of steroids and gametes is under gonadotropin support, but there is little information as to the molecular mechanisms by which these are regulated in the human. The testicular response to gonadotropin withdrawal is important for the development of effective contraceptive methods. Our objective was investigation of expression of genes in the normal human testis reflecting steroidogenesis, Sertoli cell function, and spermatogenesis after short-term gonadotropin withdrawal and the effects of activating testicular progesterone receptors. We conducted a randomized controlled trial at a research institute. Thirty healthy men participated. Subjects were randomized to no treatment or gonadotropin suppression by GnRH antagonist (cetrorelix) with testosterone (CT group) or with additional administration of the gestogen desogestrel (CTD group) for 4 wk before testicular biopsy. Gene expression was quantified by RT-PCR. Both treatment groups showed similar suppression of gonadotropins and sperm production and markedly reduced expression of steroidogenic enzymes. Addition of progestogen in the CTD group resulted in reduced expression of 5alpha-reductase type 1 compared with both controls and the CT group. Inhibin-alpha...Continue Reading

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Citations

Apr 26, 2008·Endocrine Reviews·Stephanie T PageWilliam J Bremner
Feb 13, 2016·Drug and Chemical Toxicology·Sutisa Nudmamud-ThanoiSamur Thanoi
Aug 7, 2008·Reproductive Biomedicine Online·Shadaan AbidDeepak Modi
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Apr 12, 2011·Current Opinion in Endocrinology, Diabetes, and Obesity·Stephanie T Page
Jun 9, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jing HuangShi Xi Chen

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