Direct Effect of Remifentanil and Glycine Contained in Ultiva® on Nociceptive Transmission in the Spinal Cord: In Vivo and Slice Patch Clamp Analyses

PloS One
Makoto SumieMegumu Yoshimura

Abstract

Ultiva® is commonly administered intravenously for analgesia during general anaesthesia and its main constituent remifentanil is an ultra-short-acting μ-opioid receptor agonist. Ultiva® is not approved for epidural or intrathecal use in clinical practice. Previous studies have reported that Ultiva® provokes opioid-induced hyperalgesia by interacting with spinal dorsal horn neurons. Ultiva® contains glycine, an inhibitory neurotransmitter but also an N-methyl-D-aspartate receptor co-activator. The presence of glycine in the formulation of Ultiva® potentially complicates its effects. We examined how Ultiva® directly affects nociceptive transmission in the spinal cord. We made patch-clamp recordings from substantia gelatinosa (SG) neurons in the adult rat spinal dorsal horn in vivo and in spinal cord slices. We perfused Ultiva® onto the SG neurons and analysed its effects on the membrane potentials and synaptic responses activated by noxious mechanical stimuli. Bath application of Ultiva® hyperpolarized membrane potentials under current-clamp conditions and produced an outward current under voltage-clamp conditions. A barrage of excitatory postsynaptic currents (EPSCs) evoked by the stimuli was suppressed by Ultiva®. Miniature EPS...Continue Reading

References

Sep 1, 1995·Anesthesia and Analgesia·M DershwitzB D Jamerson
May 21, 1999·The European Journal of Neuroscience·D J Maxwell, J S Riddell
Jun 18, 2002·Acta Neurologica Scandinavica·A Kostera-PruszczykK Rowińska-Marcińska
Jun 28, 2003·Science·Seifollah AhmadiHanns Ulrich Zeilhofer
Jan 14, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jennifer Y XieTodd W Vanderah
Mar 2, 2006·Anesthesiology·Martin S Angst, J David Clark
Mar 10, 2007·Intensive Care Medicine·Marie-Pierre BonnetDan Benhamou

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Software Mentioned

pCLAMP
Synaptsoft
GraphPad Prism
GraphPad
ARRIVE
Mini Analysis
Ultiva®

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