Direct effects of prolactin and dopamine on the gonadotroph response to GnRH

The Journal of Endocrinology
Helen L HendersonDomingo J Tortonese

Abstract

The intrapituitary mechanisms underlying the inhibitory actions of hyperprolactinaemia on the reproductive axis remain unclear. Previous work on primary pituitary cultures revealed combined suppressive effects of prolactin (PRL) and dopamine on the gonadotrophin response to GnRH. However, whether these effects occur directly at the level of the gonadotroph and are accompanied by changes in gene expression is still unresolved. Here, alphaT(3)-1 and LbetaT2 cells were used to investigate the effects of PRL and dopamine on gonadotrophin synthesis and release in gonadotroph monocultures under basal and GnRH-stimulated conditions. PRL receptor and dopamine receptor mRNA expressions were first determined by RT-PCR in both cell lines. Then, PRL and the dopamine agonist bromocriptine (Br), alone or in combination, were shown to block the maximal alpha-subunit and LHbeta-subunit mRNA responses to a dose-range of GnRH. The LH secretory response was differentially affected by treatments. GnRH dose-dependently stimulated LH release, with a 4-5 fold increase at 10(-8) M GnRH. Unexpectedly, PRL or Br stimulated basal LH release, with PRL, but not Br, enhancing the LH secretory response to GnRH. This effect was, however, completely blocked by...Continue Reading

Citations

Feb 25, 2010·The Journal of Veterinary Medical Science·Yuki YamamotoKazuyoshi Taya
Jun 28, 2011·The Journal of Endocrinology·Hyun Joon KimCheMyong Ko
Jun 3, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·R SalgadoA Lafuente
Sep 12, 2013·Physiology & Behavior·Milene Leivas VieiraDaniela Cristina Ceccatto Gerardin
Dec 14, 2011·Molecular and Cellular Endocrinology·M MoussaviH R Habibi
Aug 20, 2010·Biology of Reproduction·David J HodsonDomingo J Tortonese
Jun 20, 2017·The Journal of Physical Chemistry. a·Swatantra Pratap SinghSooraj Kunnikuruvan
Nov 2, 2016·Hormone Molecular Biology and Clinical Investigation·Nirja K Chaudhari, Laxmipriya P Nampoothiri
Apr 13, 2018·Reproductive Biology and Endocrinology : RB&E·Nirja ChaudhariLaxmipriya Nampoothiri
Mar 7, 2021·Scientific Reports·Jarrod McKennaPeter Temple-Smith
Aug 10, 2021·Toxicology and Applied Pharmacology·Randi GrønnestadAugustine Arukwe

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