Direct electrical detection of DNA synthesis.

Proceedings of the National Academy of Sciences of the United States of America
Nader PourmandRonald W Davis

Abstract

Rapid, sequence-specific DNA detection is essential for applications in medical diagnostics and genetic screening. Electrical biosensors that use immobilized nucleic acids are especially promising in these applications because of their potential for miniaturization and automation. Current DNA detection methods based on sequencing by synthesis rely on optical readouts; however, a direct electrical detection method for this technique is not available. We report here an approach for direct electrical detection of enzymatically catalyzed DNA synthesis by induced surface charge perturbation. We discovered that incorporation of a complementary deoxynucleotide (dNTP) into a self-primed single-stranded DNA attached to the surface of a gold electrode evokes an electrode surface charge perturbation. This event can be detected as a transient current by a voltage-clamp amplifier. Based on current understanding of polarizable interfaces, we propose that the electrode detects proton removal from the 3'-hydroxyl group of the DNA molecule during phosphodiester bond formation.

References

Aug 31, 2000·Annual Review of Biochemistry·T A Kunkel, K Bebenek
May 23, 2002·Biophysical Journal·Yuri GeorgievskiiAlexei A Stuchebrukhov
Aug 29, 2002·Quarterly Reviews of Biophysics·Lilian T C FrançaTarso B L Kist
Oct 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jurgen FritzScott R Manalis
Oct 2, 2003·Nature Biotechnology·T Gregory DrummondJacqueline K Barton
Jan 1, 1997·Annual Review of Physical Chemistry·M Gutman, E Nachliel
Mar 23, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·David B RobinsonShan X Wang
Mar 23, 2005·Trends in Biotechnology·Chunhai FanAlan J Heeger
Apr 1, 2005·Journal of Biomolecular Screening·Adrienne E DubinChris Mathes

❮ Previous
Next ❯

Citations

Aug 3, 2010·Analytical and Bioanalytical Chemistry·Ian L JonesAndreas Hierlemann
Sep 10, 2011·Nature Biotechnology·Keith Robison
Jun 12, 2013·Nature Methods·Christofer ToumazouLinglan Zhang
Apr 3, 2012·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Stuart Lindsay
Sep 20, 2011·Genome Research·Dent EarlBenedict Paten
Oct 12, 2013·PloS One·Michael S AkhrasNader Pourmand
Mar 27, 2012·Annual Review of Biochemistry·Patrik L Ståhl, Joakim Lundeberg
Jun 2, 2014·Blood Cells, Molecules & Diseases·S M HassanC L Harteveld
Feb 3, 2009·Sensors and Actuators. B, Chemical·Erik P AndersonNader Pourmand
Aug 14, 2012·Biotechnology Journal·Henrik Stranneheim, Joakim Lundeberg
Apr 8, 2015·IEEE Transactions on Bio-medical Engineering·Xiwei HuangDongping Wu
May 31, 2014·IEEE Transactions on Biomedical Circuits and Systems·Marco CrescentiniMarco Tartagni
Aug 2, 2014·Scientific Reports·Zhi XuJinfei Chen
Feb 6, 2015·BioTechniques·Nathan A TannerThomas C Evans
Aug 6, 2015·Cancer Informatics·Hongxiang FengDeruo Liu
Sep 19, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Takafumi NakagakiYasushi Sasaki
May 9, 2007·Nanotechnology·Jason ReedJames K Gimzewski
Jul 22, 2011·Nature·Jonathan M RothbergJames Bustillo
Dec 8, 2019·Clinical Chemistry·Ge Gao, David I Smith
Nov 16, 2012·Clinical Chemistry·Nardin Samuel, Thomas J Hudson
Apr 20, 2011·Lab on a Chip·Afshin Ahmadian, Helene Andersson Svahn
Feb 24, 2016·World Journal of Gastroenterology : WJG·Yasushi SasakiTakashi Tokino
Dec 17, 2008·Analytical Chemistry·Cheng FangNarayanan Balasubramanian
Aug 30, 2018·ACS Sensors·Xing SuMadoo Varma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.