PMID: 8948494Nov 14, 1996Paper

Direct electrochemical evidence for an equilibrium intermediate in the guanidine-induced unfolding of cytochrome c

Biochimica Et Biophysica Acta
T FerriR Santucci

Abstract

This paper reports a voltammetric and spectroscopic investigation of the guanidine-induced unfolding of cytochrome c at neutral pH and 25 degrees C. Electrochemical data provide direct evidence for the presence of an equilibrium intermediate (form I) strictly dependent on the denaturant concentration. The midpoint potential of form I has been determined (E1/2 = +0.010 V vs. NHE) and its structural features defined from analysis of the circular dichroism and absorbance spectroscopy data obtained under the same experimental conditions. From the correlation of electrochemical and spectroscopic data, we propose that the features detected by the intermediate conform to the molten globule state.

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Citations

May 11, 2013·Analytical and Bioanalytical Chemistry·Maria BosserdtFrieder W Scheller
Oct 27, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paola CaroppiRoberto Santucci
Aug 15, 2001·European Journal of Biochemistry·F SinibaldiR Santucci
Apr 26, 2013·Cellular and Molecular Life Sciences : CMLS·Sobia ZaidiFaizan Ahmad
Dec 29, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Daniela DeriuNicola Rosato
Mar 10, 2009·Journal of Molecular Biology·Megan C ThielgesFloyd E Romesberg
Oct 3, 2001·Journal of Molecular Biology·A K Bhuyan, J B Udgaonkar
Feb 14, 2006·Journal of Molecular Biology·Ramil F LatypovHeinrich Roder
Aug 7, 2003·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Hainer Wackerbarth, Peter Hildebrandt
Mar 25, 2011·Metallomics : Integrated Biometal Science·Md Khurshid Alam KhanFaizan Ahmad
Jun 30, 2019·International Journal of Biological Macromolecules·Roberto SantucciLaura Fiorucci
Jul 19, 2000·Archives of Biochemistry and Biophysics·R SantucciF Ascoli
Jan 20, 1998·Biochimica Et Biophysica Acta·T PinedaM Blázquez
May 20, 2004·Journal of Inorganic Biochemistry·Elisa SantoniGiulietta Smulevich
Mar 11, 2011·Biochemistry·Shigeyoshi NakamuraShun-ichi Kidokoro
Oct 14, 2017·Chemical Reviews·Damián Alvarez-PaggiDaniel H Murgida
Oct 12, 2000·Biochemistry·R SantucciA Desideri
Oct 30, 2003·Journal of Agricultural and Food Chemistry·Kohei SurugaTadatake Oku
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Sep 2, 2006·Analytical Chemistry·Liang-Hong Guo, Na Qu
Oct 18, 2006·Biochemistry·Susie Y Dai, Michael C Fitzgerald
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Nov 13, 2004·Journal of the American Chemical Society·Susumu UchiyamaYuji Kobayashi

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