Direct electrochemical immunoassay based on immobilization of protein-magnetic nanoparticle composites on to magnetic electrode surfaces by sterically enhanced magnetic field force

Biotechnology Letters
Dianping TangYaqin Chai

Abstract

A direct electrochemical immunoassay system based on the immobilization of alpha-1-fetoprotein antibody (anti-AFP), as a model system, on the surface of core-shell Fe(2)O(3)/Au magnetic nanoparticles (MNP) has been demonstrated. To fabricate such an assay system, anti-AFP was initially covalently immobilized on to the surface of core-shell Fe(2)O(3)/Au MNP. Anti-AFP-modified MNP (bio-nanoparticles) were then attached to the surface of carbon paste electrode with the aid of a permanent magnet. The performance and factors influencing the performance of the resulting immunosensor were studied. alpha-1-Fetoprotein antigen was directly determined by the change in current or potential before and after the antigen-antibody reaction versus saturated calomel electrode. The electrochemical immunoassay system reached 95% of steady-state potential within 2 min and had a sensitivity of 25.8 mV. The linear range for AFP determination was from 1 to 80 ng AFP ml(-1) with a detection limit of 0.5 ng AFP ml(-1). Moreover, the direct electrochemical immunoassay system, based on a functional MNP, can be developed further for DNA sensor and enzyme biosensor.

References

Dec 18, 2001·Biosensors & Bioelectronics·Nirmalya K Chaki, K Vijayamohanan
Nov 15, 2002·Electrophoresis·Joseph WangMadhu Prakash Chatrathi
Mar 12, 2003·Advanced Drug Delivery Reviews·Kiichi SatoTakehiko Kitamori
Apr 26, 2005·Journal of Colloid and Interface Science·Madhuri MandalTarasankar Pal

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Citations

Jan 1, 2009·Sensors·Jana DrbohlavovaJaromir Hubalek
Jan 27, 2009·Biosensors & Bioelectronics·Caifeng DingShusheng Zhang
Mar 7, 2008·Sensors·Dorothee GrieshaberErik Reimhult
Nov 11, 2011·Analytical Chemistry·Hirokazu NagaokaNoriyuki Ohnishi

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