Direct electrochemistry of hemoglobin in layer-by-layer films with poly(vinyl sulfonate) grown on pyrolytic graphite electrodes

Bioelectrochemistry
Liwen Wang, Naifei Hu

Abstract

Stable layer-by-layer electroactive films were grown on pyrolytic graphite (PG) electrodes by alternate adsorption of layers of polyanionic poly(vinyl sulfonate) (PVS) and positively charged hemoglobin (Hb) from their aqueous solutions. Cyclic voltammetry (CV) of [PVS/Hb]n films showed a pair of well-defined and nearly reversible peaks at about - 0.28 V vs. SCE at pH 5.5, characteristic of Hb heme Fe(III)/Fe(II) redox couple. The process of (PVS/Hb) bilayer growth was monitored and confirmed by CV, X-ray photoelectron spectroscopy (XPS) and UV-Vis spectroscopy. While the amount of Hb adsorbed in each bilayer was the same, the amount of electroactive Hb in each bilayer decreased dramatically with increase of the number of bilayer, and electroactivity was just extended to 8 [PVS/Hb] bilayers. CVs of [PVS/Hb]8 films maintained stable in buffers containing no Hb. Positions of Soret band of Hb in [PVS/Hb]n films grown on transparent glass slides suggest that Hb in the films keeps its secondary structure similar to its native state in a wide pH range. Trichloroacetic acid and nitrite were catalytically reduced by [PVS/Hb]8 films with significant lowering of the electrode potential required.

Citations

Apr 17, 2007·Journal of Biochemical and Biophysical Methods·Ling MaZhijiang Rong
Oct 16, 2007·Biochemical and Biophysical Research Communications·Meera ParthasarathyM I Khan
Aug 16, 2005·Advances in Colloid and Interface Science·Frieder W SchellerUlla Wollenberger
Jun 10, 2006·Journal of Biochemical and Biophysical Methods·Hui-Hong Liu, Guo-Lin Zou
Jan 27, 2004·Biochimica Et Biophysica Acta·Li Shen, Naifei Hu
Jan 3, 2006·International Journal of Biological Macromolecules·Wenjie LiuRong Guo
Sep 25, 2007·International Journal of Biological Macromolecules·Wenjie LiuRong Guo
Mar 19, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Pingli HeJames F Rusling
Sep 1, 2006·The Journal of Physical Chemistry. B·Yonghong NiXianwen Wei
Nov 17, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Hongyun LiuNaifei Hu
Sep 21, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Sheng-Fu WangKwok-Yin Wong

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