Direct electrochemistry of hemoglobin in PHEA and its catalysis to H2O2

Biosensors & Bioelectronics
Qing LuShengshui Hu

Abstract

Hemoglobin (Hb) was immobilized on glassy carbon (GC) electrode by a kind of synthetic water-soluble polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide] (PHEA). A pair of well-defined and quasi-reversible cyclic voltammetric peaks was achieved, which reflected the direct electron-transfer of the Fe(III)/Fe(II) couple of Hb. The formal potential (E degrees'), the apparent coverage (Gamma(*)) and the electron-transfer rate constant (k(s)) were calculated by integrating cyclic voltammograms experimental data. Scanning electron microscopy (SEM) demonstrated the morphology of Hb-PHEA film very different from the Hb and PHEA films. Ultraviolet visible (UV-vis) spectroscopy showed Hb in PHEA film remained its secondary structure similar to the native state. In respect that the immobilized protein remained its biocatalytic activity to the reduction of hydrogen peroxide (H(2)O(2)), a kind of mediator-free biosensor for H(2)O(2) could be developed. The apparent Michaelis-Menten constant (K(m)(app)) was estimated to be 18.05 microM. The biosensor exhibited rapid electrochemical response and good stability. Furthermore, uric acid (UA), ascorbic acid (AA) and dopamine (DA) had little interferences with the amperometric signal of H(2...Continue Reading

References

Dec 12, 2001·Current Opinion in Chemical Biology·T G Spiro, A A Jarzecki
Apr 25, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gennara CavallaroStefano Salmaso
Nov 11, 2003·Biomacromolecules·Ann-Christine Albertsson, Indra K Varma
May 26, 2004·International Journal of Pharmaceutics·V R SinhaA Trehan

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Citations

Apr 21, 2011·Bioprocess and Biosystems Engineering·Changhua LiuZongfang Wu
Jun 18, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zhan WangYuan-Di Zhao
Mar 14, 2008·Bioelectrochemistry·Yanxia XuShengshui Hu
Feb 19, 2011·Biomedit︠s︡inskai︠a︡ khimii︠a︡·V V ShumiantsevaA I Archakov

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