Direct electrochemistry of hemoglobin on carbonized titania nanotubes and its application in a sensitive reagentless hydrogen peroxide biosensor

Biosensors & Bioelectronics
Chunxian GuoPei Kang Shen

Abstract

Carbonized TiO(2) nanotubes (TNT/C) prepared by carbonization with organic polymers possess advantages combined from high conductivity of carbon and nanostructure of TiO(2) nanotubes. The material was used as a supporting matrix to immobilize a redox protein, hemoglobin (Hb), to explore its direct electron transfer ability. The apparent heterogeneous electron transfer rate constant (k(ET)) of Hb on TNT/C is 108s(-1), which is much higher than that in the reported works, demonstrating excellent direct electrochemistry behavior. The TNT/C-Hb modified glassy carbon electrode (GCE) demonstrates significant electrocatalytic activity for reduction of hydrogen peroxide with a small apparent Michaelis-Menten constant (87.5 microM). The TNT/C-Hb based H(2)O(2) sensor has a low detection limit (0.92 microM), fast response time (3s) and high dynamic response range (10(-6) to 10(-4)M), a much better performance than the reported works. These results demonstrate that a direct electrochemistry behavior can be significantly enhanced through simple carbon coating on a nanostructured material for higher reaction surface area and better conductivity. This work suggests that Hb-immobilized TNT/C has potential applications in a sensitive H(2)O(2) ...Continue Reading

References

Oct 2, 2007·Journal of Colloid and Interface Science·Renyun ZhangKwok-Keung Shiu

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Citations

Jul 3, 2010·Nanoscale Research Letters·Jinping LiuZhihong Zhu
Jan 7, 2014·Biosensors & Bioelectronics·Mehdi BaghayeriMoslem Mansour Lakouraj
Oct 31, 2012·International Journal of Molecular Sciences·Larissa M Uusitalo, Nadine Hempel
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Jun 13, 2014·Chemical Reviews·Jing Bai, Baoxue Zhou

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