Direct electron transfer of hemoglobin in layered alpha-zirconium phosphate with a high thermal stability

Analytical Biochemistry
Yuge LiuJun-Jie Zhu

Abstract

A heme protein hemoglobin (Hb) was reacted with preexfoliated layered alpha-zirconium phosphate (alpha-ZrP) platelets. An X-ray diffraction (XRD) pattern of small range showed that the exfoliated alpha-ZrP platelets reassembled after the addition of Hb molecules, with the protein intercalated between the layers. UV-Vis and Fourier transform infrared (FTIR) spectra analysis displayed that no significant denaturation occurred to the intercalated protein. The bioactivity of Hb was also investigated by testing the electrochemical properties of the Hb/alpha-ZrP composite. Results showed that the intercalation of Hb into the layered material not only improved the thermal stability of Hb but also enhanced the direct electron transfer ability between protein molecules and electrode. The protein still showed bioactivity after treatment at a temperature as high as 85 degrees C. A pair of well-defined redox peaks at approximately -0.37 and -0.32V was observed on the cyclic voltammograms (CVs) of the Hb/alpha-ZrP composite modified electrode, and the electrode reactions showed a surface-controlled process with a single proton transfer. The resultant biosensor constructed by the Hb/alpha-ZrP composite displayed an excellent response to the ...Continue Reading

References

Feb 12, 1998·Electrophoresis·E V TrushinaC T McMurray
Sep 17, 2004·Journal of the American Chemical Society·J Justin GoodingD Brynn Hibbert
Sep 21, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Sheng-Fu WangKwok-Yin Wong
Jan 4, 2007·Analytical and Bioanalytical Chemistry·Yuge LiuWenhua Hou

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Citations

Apr 21, 2011·Bioprocess and Biosystems Engineering·Changhua LiuZongfang Wu
Aug 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Changzhi ZhaoKui Jiao
Jan 1, 2011·Analytical Methods : Advancing Methods and Applications·Shengwei TanLi Liu

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