Direct estimate of the mutation rate and the distribution of fitness effects in the yeast Saccharomyces cerevisiae.

Genetics
Dominika M WlochRyszard Korona

Abstract

Estimates of the rate and frequency distribution of deleterious effects were obtained for the first time by direct scoring and characterization of individual mutations. This was achieved by applying tetrad analysis to a large number of yeast clones. The genomic rate of spontaneous mutation deleterious to a basic fitness-related trait, that of growth rate, was U = 1.1 x 10(-3) per diploid cell division. Extrapolated to the fruit fly and humans, the per generation rate would be 0.074 and 0.92, respectively. This is likely to be an underestimate because single mutations with selection coefficients s < 0.01 could not be detected. The distribution of s > or = 0.01 was studied both for spontaneous and induced mutations. The latter were induced by ethyl methanesulfonate (EMS) or resulted from defective mismatch repair. Lethal changes accounted for approximately 30-40% of the scored mutations. The mean s of nonlethal mutations was fairly high, but most frequently its value was between 0.01 and 0.05. Although the rate and distribution of very small effects could not be determined, the joint share of such mutations in decreasing average fitness was probably no larger than approximately 1%.

References

May 23, 1991·Nature·A S Kondrashov, J F Crow
May 23, 1991·Nature·V PerrotM Valéro
Nov 1, 1987·Molecular and Cellular Biology·J H McCuskerJ E Haber
Sep 1, 1984·Mutation Research·G A Sega
Apr 1, 1995·Genetical Research·N H Barton
Feb 15, 1996·Genes & Development·G T MarsischkyR Kolodner
Jun 15, 1996·Genes & Development·R Kolodner
Dec 1, 1996·Genetics·P D Keightley
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·P D Keightley, A Caballero
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·S A ShabalinaA S Kondrashov
Apr 30, 1998·Genetics·J W DrakeJ F Crow
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·J D FryS V Nuzhdin
Feb 9, 1999·Nature·A Eyre-Walker, P D Keightley
Jul 10, 1999·Science·T GalitskiG R Fink
Oct 8, 1999·Genetics·P D Keightley, A Eyre-Walker
Jan 11, 2000·Molecular & General Genetics : MGG·K D EntianA Hinnen
Feb 26, 2000·Genetical Research·A García-DoradoA Caballero
Mar 25, 2000·Science·M D AdamsJ C Venter
Sep 27, 2000·Evolution; International Journal of Organic Evolution·L L VassilievaM Lynch
Oct 13, 2000·Science·P D Keightley, A Eyre-Walker
Feb 22, 2001·Science·J C VenterX Zhu
Mar 10, 2001·Nature·E S LanderUNKNOWN International Human Genome Sequencing Consortium
Mar 27, 2001·Nature Reviews. Genetics·J F Crow
May 1, 1964·Mutation Research·H J MULLER
Nov 1, 1956·Proceedings of the National Academy of Sciences of the United States of America·N E MortonH J Muller
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M W FeldmanL D Brooks
Dec 1, 1966·Genetics·M Kimura, T Maruyama
Jun 1, 1995·Evolution; International Journal of Organic Evolution·Cheryl D Jenkins, Mark Kirkpatrick
Jun 1, 1998·Evolution; International Journal of Organic Evolution·Michael LynchMatthew Giorgianni
Dec 1, 1999·Evolution; International Journal of Organic Evolution·Ryszard Korona
Jun 1, 1999·Evolution; International Journal of Organic Evolution·Michael LynchJohn Willis
Dec 1, 1993·Evolution; International Journal of Organic Evolution·W GabrielR Bürger

❮ Previous
Next ❯

Citations

Oct 18, 2006·Journal of Molecular Evolution·Joshua B PlotkinHunter B Fraser
Jun 23, 2004·Research in Microbiology·Ryszard Korona
Jun 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michael LynchW Kelley Thomas
Jan 30, 2002·Yeast
May 4, 2005·Protein Expression and Purification·Mei M Whittaker, James W Whittaker
Feb 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Julie Akiko HeckEric Alani
Apr 22, 2003·Evolution; International Journal of Organic Evolution·Peter D Keightley, Michael Lynch
May 14, 2003·Fungal Genetics and Biology : FG & B·A ForcheP T Magee
Jan 19, 2007·Nature Reviews. Genetics·J Arjan G M de Visser, Santiago F Elena
Jan 24, 2007·Genetics·Jessica A Hill, Sarah P Otto
Jun 16, 2017·Evolutionary Applications·Vincent L CannataroColette M St Mary
Aug 13, 2013·Genetics·Hong-An LongRebecca A Zufall
Jul 22, 2005·Journal of Evolutionary Biology·J M RosaA García-Dorado
Jul 27, 2018·Microbiology and Molecular Biology Reviews : MMBR·Bram Van den BerghJan Michiels
May 31, 2003·Nature Reviews. Genetics·Santiago F Elena, Richard E Lenski
Jan 7, 2014·Genetics·Alan J HerrBradley D Preston
Sep 22, 2020·Evolutionary Applications·Lauren M F MerloCarlo C Maley
Sep 7, 2014·Genetics·Benjamin H Good, Michael M Desai
Mar 13, 2015·Genetics·Daniel P RiceMichael M Desai
Nov 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Piotr Sliwa, Ryszard Korona
Jul 20, 2007·Nature Reviews. Genetics·Adam Eyre-Walker, Peter D Keightley
Jul 23, 2003·Genetics·Ned S WingreenEdward C Cox
Sep 21, 2016·Genetics·Benjamin H Good, Michael M Desai
Sep 25, 2003·Evolution; International Journal of Organic Evolution·Xu-Sheng Zhang, William G Hill
Mar 12, 2021·G3 : Genes - Genomes - Genetics·Saurin Bipin ParikhAnne-Ruxandra Carvunis
Jul 27, 2002·Annual Review of Microbiology·Leah E CowenLinda M Kohn
May 29, 2009·Evolution; International Journal of Organic Evolution·Angelina Fudala, Ryszard Korona

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.