PMID: 2105657Jan 11, 1990Paper

Direct evaluation of acidification by rat testis and epididymis: role of carbonic anhydrase

The American Journal of Physiology
C R Caflisch, T D DuBose

Abstract

The present experiments have employed microelectrode techniques (pH and PCO2) and microcalorimetry (total CO2 concentration) to define parameters of acidification in specific structures of the rat testis and epididymis during control conditions and after administration of the carbonic anhydrase inhibitor acetazolamide (20 or 50 mg/kg). Values for in situ pH during control conditions in seminiferous tubules (ST; 6.96 +/- 0.01), proximal caput (PCP; 6.62 +/- 0.01), middle caput (MCP; 6.59 +/- 0.01), middle corpus (MCR; 7.10 +/- 0.02), and proximal cauda epididymidis (PCD; 6.85 +/- 0.01) were significantly more acidic than in testicular artery (TA; 7.36 +/- 0.01) or systemic arterial blood (SAB; 7.40 +/- 0.01) and did not change significantly after acetazolamide. In situ partial pressure of CO2 (PCO2) in TA (52.2 +/- 0.6 mmHg), ST (52.3 +/- 0.4 mmHg), PCP (52.9 +/- 0.4 mmHg), MCP (53.0 +/- 0.7 mmHg), MCR (53.4 +/- 0.4 mmHg), and PCD (52.4 +/- 0.4 mmHg) were indistinguishable from each other, but all values were significantly higher than SAB PCO2 (39.2 +/- 0.5 mmHg). Acetazolamide increased in situ PCO2 significantly in all structures except the MCR. The total CO2 concentration in normal ST fluid (10.7 +/- 0.5 mM) was significantly...Continue Reading

References

May 1, 1976·Biology of Reproduction·M J FreeR A Jaffe
Mar 1, 1978·Journal of Reproduction and Fertility·N Levine, H Kelly
Aug 1, 1978·Kidney International·C R CaflischN W Carter
Aug 1, 1978·The Journal of Clinical Investigation·T D DuBoseN W Carter
Oct 1, 1978·Analytical Biochemistry·L R Pucacco, N W Carter
Apr 1, 1975·Analytical Chemistry·G G VurekR Corsey
Nov 1, 1984·Journal of Reproduction and Fertility·T T Turner
Apr 1, 1982·Physiological Reviews·G M Waites, R T Gladwell
Dec 1, 1980·The Journal of Physiology·C L Au, P Y Wong
Mar 1, 1960·The American Journal of Physiology·G W SALISBURYR G CRAGLE

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Citations

May 23, 2001·Biology of Reproduction·C M Herak-KrambergerI Sabolic
Oct 10, 2013·The Journal of Membrane Biology·Raquel L BernardinoPedro F Oliveira
Feb 22, 2001·American Journal of Physiology. Renal Physiology·C BagnisS Breton
Dec 12, 2002·American Journal of Physiology. Cell Physiology·Corinne Isnard-BagnisSylvie Breton
May 12, 2000·American Journal of Physiology. Renal Physiology·S BretonD Brown
Mar 30, 2007·American Journal of Physiology. Cell Physiology·Nicolas Da SilvaSylvie Breton
Aug 11, 2010·The Journal of Membrane Biology·Luís RatoPedro F Oliveira
Dec 21, 2000·American Journal of Physiology. Cell Physiology·P J Smith, J Trimarchi
Aug 15, 2018·Frontiers in Cell and Developmental Biology·Lis C Puga MolinaMariano G Buffone

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