Direct evidence for activated CD8+ T cell transmigration across portal vein endothelial cells in liver graft rejection

Journal of Gastroenterology
Taro KariyaKenjiro Matsuno

Abstract

Lymphocyte recruitment into the portal tract is crucial not only for homeostatic immune surveillance but also for many liver diseases. However, the exact route of entry for lymphocytes into portal tract is still obscure. We investigated this question using a rat hepatic allograft rejection model. A migration route was analyzed by immunohistological methods including a recently developed scanning electron microscopy method. Transmigration-associated molecules such as selectins, integrins, and chemokines and their receptors expressed by hepatic vessels and recruited T-cells were analyzed by immunohistochemistry and flow cytometry. The immunoelectron microscopic analysis clearly showed CD8β(+) cells passing through the portal vein (PV) endothelia. Furthermore, the migrating pathway seemed to pass through the endothelial cell body. Local vascular cell adhesion molecule-1 (VCAM-1) expression was induced in PV endothelial cells from day 2 after liver transplantation. Although intercellular adhesion molecule-1 (ICAM-1) expression was also upregulated, it was restricted to sinusoidal endothelia. Recipient T-cells in the graft perfusate were CD25(+)CD44(+)ICAM-1(+)CXCR3(+)CCR5(-) and upregulated α4β1 or αLβ2 integrins. Immunohistochemis...Continue Reading

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Citations

Feb 15, 2019·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Liwen LinTodd V Brennan
Mar 1, 2019·Transplantation·Sulemon ChaudhryAdam Griesemer
Jan 9, 2021·Transplantation·Xinle WangCatia T Perciani

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Methods Mentioned

BETA
scanning electron microscopy
light microscopy
Light
imaging technique

Software Mentioned

FlowJo
Axiovision

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