Direct evidence for control of the pheromone-inducible prgQ operon of Enterococcus faecalis plasmid pCF10 by a countertranscript-driven attenuation mechanism.

Journal of Bacteriology
Christopher M JohnsonG M Dunny

Abstract

The mating response of Enterococcus faecalis cells carrying the conjugative plasmid pCF10 is controlled by multiple regulatory circuits. Initiation of transcription of the prgQ conjugation operon is controlled by the peptide receptor protein PrgX; binding of the pheromone peptide cCF10 to PrgX abolishes PrgX repression, while binding of the inhibitor peptide iCF10 enhances repression. The results of molecular analysis of prgQ transcripts and genetic studies suggested that the elongation of prgQ transcripts past a putative terminator (IRS1) may be controlled by the interaction of nascent prgQ mRNAs with a small antisense RNA (Anti-Q) encoded within prgQ. Direct evidence for interaction of these RNAs, as well as the resulting effects on readthrough of prgQ transcription, has been limited. Here we report the results of experiments that (i) determine the inherent termination properties of prgQ transcripts in the absence of Anti-Q; (ii) determine the direct effects of the interaction of Anti-Q with nascent prgQ transcripts in the absence of complicating effects of the PrgX protein; and (iii) begin to dissect the structural components involved in these interactions. The results confirm the existence of alternative terminating and ant...Continue Reading

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Citations

May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anushree ChatterjeeWei-Shou Hu
Sep 6, 2012·The Journal of Biological Chemistry·Ronnie P-A BerntssonBert Poolman
Mar 6, 2012·Journal of Bacteriology·Ann M StevensKendra P Rumbaugh
Apr 13, 2010·Journal of Bacteriology·Susanne Häussler, Matthew R Parsek
Sep 9, 2011·PLoS Computational Biology·Che-Chi ShuDoraiswami Ramkrishna
May 15, 2012·Chemical Engineering Science·Che-Chi ShuDoraiswami Ramkrishna
Sep 30, 2014·Plasmid·Margarida SaramagoSusana Domingues
Apr 23, 2013·International Journal of Medical Microbiology : IJMM·Guido WernerKeith Weaver
Oct 15, 2013·FEMS Microbiology Reviews·Laura C Cook, Michael J Federle
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Christopher M JohnsonGary M Dunny
Sep 21, 2013·Annual Review of Genetics·Gary M Dunny
Oct 28, 2016·Molecular Microbiology·Minny BhattyPeter J Christie
May 22, 2016·Applied and Environmental Microbiology·Arpan BandyopadhyayWei-Shou Hu
Mar 30, 2016·Journal of Bacteriology·Gary M Dunny, Ronnie Per-Arne Berntsson
Apr 3, 2020·PLoS Pathogens·Amy J SterlingJames S G Dooley
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Jun 14, 2019·Frontiers in Microbiology·Verena KohlerElisabeth Grohmann
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