PMID: 7537182Apr 1, 1995Paper

Direct evidence for hydroxyl radical-induced damage to nucleic acids by chromium(VI)-derived species: implications for chromium carcinogenesis

Carcinogenesis
M J Molyneux, M J Davies

Abstract

Reduction of Cr(VI) by NADH and NADPH has been shown to yield Cr(V) species, which have been detected by electron paramagnetic resonance (EPR) spectroscopy. The fine structure on the EPR signal of the Cr(V) species is consistent with the presence of two NAD(P)H ligands in a square-pyramidal arrangement with a single oxygen (oxo) group at the apex. Neither this species nor the initial Cr(VI) complex damage DNA components as evidenced by the lack of effect of these compounds on the optical and EPR signals of the Cr(VI) and Cr(V) species respectively. Addition of hydrogen peroxide to the Cr(V) species is shown to result in the formation of a further transient EPR signal, the parameters of which are consistent with an assignment to a Cr(V)-peroxide complex. Inclusion of the spin trap 5,5-dimethyl-1-pyrroline-N-oxide in this system demonstrates that hydroxyl radicals are also generated, possibly via the decomposition of the peroxide complex. Inclusion of DNA components in this system together with the spin trap 2-methyl-2-nitrosopropane results in the detection of base- and sugar-derived radicals; the characteristic EPR signals of these species have allowed both the identification of these species and their mechanism of formation to...Continue Reading

Citations

May 15, 2009·International Journal of Environmental Research and Public Health·Athanasios ValavanidisKonstantinos Fiotakis
Feb 27, 2007·Free Radical Biology & Medicine·Griselda R BorthiryCharles R Myers
May 24, 2006·Free Radical Biology & Medicine·Joseph MesserAnatoly Zhitkovich
May 1, 2012·Free Radical Biology & Medicine·Charles R Myers
Feb 19, 2008·Journal of Inorganic Biochemistry·Griselda R BorthiryCharles R Myers
Nov 1, 2007·Chemical Research in Toxicology·Konstantin Salnikow, Anatoly Zhitkovich

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