Direct evidence of generation and accumulation of β-sheet-rich prion protein in scrapie-infected neuroblastoma cells with human IgG1 antibody specific for β-form prion protein.

The Journal of Biological Chemistry
Toshiya KubotaKazuhisa Sugimura

Abstract

We prepared β-sheet-rich recombinant full-length prion protein (β-form PrP) (Jackson, G. S., Hosszu, L. L., Power, A., Hill, A. F., Kenney, J., Saibil, H., Craven, C. J., Waltho, J. P., Clarke, A. R., and Collinge, J. (1999) Science 283, 1935-1937). Using this β-form PrP and a human single chain Fv-displaying phage library, we have established a human IgG1 antibody specific to β-form but not α-form PrP, PRB7 IgG. When prion-infected ScN2a cells were cultured with PRB7 IgG, they generated and accumulated PRB7-binding granules in the cytoplasm with time, consequently becoming apoptotic cells bearing very large PRB7-bound aggregates. The SAF32 antibody recognizing the N-terminal octarepeat region of full-length PrP stained distinct granules in these cells as determined by confocal laser microscopy observation. When the accumulation of proteinase K-resistant PrP was examined in prion-infected ScN2a cells cultured in the presence of PRB7 IgG or SAF32, it was strongly inhibited by SAF32 but not at all by PRB7 IgG. Thus, we demonstrated direct evidence of the generation and accumulation of β-sheet-rich PrP in ScN2a cells de novo. These results suggest first that PRB7-bound PrP is not responsible for the accumulation of β-form PrP aggr...Continue Reading

References

Sep 1, 1988·European Journal of Biochemistry·E TurkS B Prusiner
Dec 1, 1983·Cell·S B PrusinerG G Glenner
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K M PanF E Cohen
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M RogersS B Prusiner
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·R A WilliamsonD R Burton
Oct 10, 1998·Journal of Virology·R A WilliamsonD R Burton
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·S B Prusiner
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·R ZahnK Wüthrich
May 11, 2004·The Journal of Biological Chemistry·Vincent BeringueSimon Hawke
Dec 25, 2004·The Journal of Biological Chemistry·Cécile FéraudetJacques Grassi
Sep 15, 2005·The Journal of Biological Chemistry·Mark KristiansenSarah J Tabrizi
Nov 8, 2005·Journal of Biochemistry·Takayuki HamasakiKazuhisa Sugimura
Jul 5, 2006·The European Journal of Neuroscience·Joanna PankiewiczThomas Wisniewski
Jul 29, 2006·Journal of Virology·Ryuichiro AtarashiShigeru Katamine
Sep 20, 2006·The Journal of Biological Chemistry·Paula SaáClaudio Soto
Jan 11, 2007·The Journal of Biological Chemistry·Remo GerberWilliam James
Jul 21, 2007·Microbiology and Immunology·Masahide UryuMotohiro Horiuchi
Feb 27, 2008·Journal of Neurochemistry·Emiliano BiasiniDavid A Harris
Aug 8, 2008·The Biochemical Journal·Sabrina CronierJonathan D F Wadsworth
Oct 7, 2008·European Journal of Cell Biology·Nathalie M VeithAlexander Bürkle
Jan 23, 2009·Prion·Ken SasakiKazuyuki Akasaka
Nov 17, 2009·PloS One·Emiliano BiasiniRoberto Chiesa
Feb 24, 2010·The Journal of Biological Chemistry·Yuko Ushiki-KakuTakashi Yokoyama
Jun 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Larry H StankerStanley B Prusiner
Jul 6, 2010·Archives of Biochemistry and Biophysics·Eriko SasamoriYoshiro Hanyu
Jul 22, 2010·Prion·Robert HnaskoLarry H Stanker
Dec 29, 2010·PloS One·Laura D'CastroJonathan D F Wadsworth
Jan 8, 2011·The Journal of Biological Chemistry·Zaira E Arellano AnayaDidier Vilette
Apr 21, 2011·Nature Communications·R GooldS J Tabrizi

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Citations

Sep 21, 2013·International Journal of Molecular Sciences·Liang HuangHoward J Federoff
Aug 5, 2016·Scientific Reports·Changyoun KimSeung-Jae Lee
Dec 24, 2014·Chemical Communications : Chem Comm·Bin WangBingqian Xu

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