Direct evidence of pancreatic tissue oxygenation during preservation by the two-layer method

Transplantation
S MatsumotoY Saitoh

Abstract

The purpose of this study was to measure directly tissue oxygen tension (PO2) of the ischemically damaged pancreas graft during preservation by the two-layer (University of Wisconsin solution/perfluoro-chemical) method at 20 degrees C using Clark-type polarographic oxygen electrodes and to make clear the relationship of tissue PO2 and ATP levels during preservation and graft survival after transplantation in a canine model. During warm ischemia, tissue PO2 and ATP were dramatically decreased. Tissue PO2 continued to decrease, and tissue ATP levels were not elevated during simple storage in oxygenated University of Wisconsin solution at 20 degrees C. On the contrary, during preservation by the two-layer method at 20 degrees C, tissue PO2 promptly increased and reached about 50% of the preprocurement level at 30 min of preservation. Then, tissue PO2 was maintained this level throughout the preservation period. Tissue ATP levels were still low in the first 1 hr, but after that tissue ATP increased in a time-dependent manner. Consequently, the two-layer method at 20 degrees C could restore the viability of pancreas damaged by warm ischemia during 3- and 5-hr preservation. We conclude that the ischemically damaged pancreas grafts we...Continue Reading

References

Feb 1, 1988·Transplantation·G M AbounaJ S Najarian
Jun 1, 1986·The Surgical Clinics of North America·D E Pegg

❮ Previous
Next ❯

Citations

Jun 7, 2008·Journal of Hepato-biliary-pancreatic Surgery·Hirofumi Noguchi, Shinichi Matsumoto
Aug 26, 2006·Cell and Tissue Banking·Guangming ZhangJo-Anna Reems
Dec 21, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Shinichi MatsumotoR Brian Stevens
Jan 26, 2010·Transplantation·Heide BrandhorstDaniel Brandhorst
Apr 16, 2010·Transplantation·Sarah A Hosgood, Michael L Nicholson
Dec 21, 2010·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Sarah A HosgoodMichael L Nicholson
Jul 9, 2010·World Journal of Gastroenterology : WJG·Yasuhiro Fujino
Jul 29, 2008·Current Opinion in Organ Transplantation·Reto M BaertschigerPhilippe Morel
Dec 24, 2005·Expert Opinion on Biological Therapy·Shinichi MatsumotoCamillo Ricordi
Jun 19, 2013·Transplantation Proceedings·M C GiovialeA I Lo Monte
Jul 22, 2009·Transplant International : Official Journal of the European Society for Organ Transplantation·Heide BrandhorstDaniel Brandhorst
Jul 11, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H IchiiC Ricordi
Feb 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H NoguchiS Matsumoto
Nov 22, 2005·Transplantation Proceedings·K K PapasE S Avgoustiniatos
May 31, 2011·Transplantation Proceedings·M C GiovialeA I Lo Monte
Apr 6, 2018·Pancreas·Hirotake KomatsuYoko Mullen
Jul 17, 2012·Cell Transplantation·Bashoo NaziruddinMarlon F Levy
Oct 8, 2005·Transplantation·Doug O'GormanJonathan R T Lakey
Jun 27, 2017·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Shinya OkumuraShinji Uemoto
May 19, 2001·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·A DeRooverM D'Silva
Oct 20, 2007·Proceedings·Shinichi MatsumotoMarlon Levy
Mar 17, 2005·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Shinichi Matsumoto
Jul 5, 2011·The International Journal of Artificial Organs·Maria Concetta GiovialeAttilio Ignazio Lo Monte

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.